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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Renal GLUT1 reduction depends on angiotensin-converting enzyme inhibition in diabetic hypertensive rats

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Autor(es):
da Silva, Ariel S. [1] ; Dias, Lucinara D. [1] ; Borges, Julia F. [1] ; Markoski, Melissa M. [1] ; de Souza, Martina S. [1, 2] ; Irigoyen, Maria C. [1, 3] ; Machado, Ubiratan F. [4] ; Schaan, Beatriz D. [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Fundacao Univ Cardiol IC FUC, Inst Cardiol Rio Grande Sul, Porto Alegre, RS - Brazil
[2] Univ Fed Rio Grande Sul UFRGS, Div Endocrine, Hosp Clin Porto Alegre HCPA, Porto Alegre, RS - Brazil
[3] Univ Sao Paulo, Unidade Hipertensao, Inst Coracao INCOR, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Physiol & Biophys, Inst Ciencia Biomed USP, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Life Sciences; v. 92, n. 24-26, p. 1174-1179, JUL 10 2013.
Citações Web of Science: 2
Resumo

Aims: Angiotensin-converting enzyme (ACE) inhibitors are used in diabetic kidney disease to reduce systemic/intra-glomerular pressure. The objective of this study was to investigate whether reducing blood pressure (BP) could modulate renal glucose transporter expression, and urinary markers of diabetic nephropathy in diabetic hypertensive rats treated with ramipril or amlodipine. Main methods: Diabetes was induced in spontaneously-hypertensive rats (similar to 210 g) by streptozotocin (50 mg/kg). Thirty days later, animals received ramipril 15 mu g/kg/day (R, n = 10), or amlodipine 10 mg/kg/day (A, n = 8,) or water (C, n = 10) by gavage. After 30-day treatment, body weight, glycaemia, urinary albumin and TGF-beta 1 (enzyme-linked immunosorbent assay) and BP (tail-cuff pressure method) were evaluated. Kidneys were removed for evaluation of renal cortex glucose transporters (Western blotting) and renal tissue ACE activity (fluorometric assay). Key findings: After treatments, body weight (p = 0.77) and glycaemia (p = 0.22) were similar among the groups. Systolic BP was similarly reduced (p < 0.001) in A and R vs. C (172.4 +/- 3.2; 186.7 +/- 3.7 and 202.2 +/- 4.3 mm Hg; respectively). ACE activity (C: 0.903 +/- 0.086; A: 0.654 +/- 0.025, and R: 0.389 +/- 0.057 mU/mg), albuminuria (C: 264.8 +/- 15.4; A: 140.8 +/- 13.5 and R: 102.8 +/- 6.7 mg/24 h), and renal cortex GLUM content (C: 46.81 +/- 4.54; A: 40.30 +/- 5.39 and R: 26.89 +/- 0.79 AU) decreased only in R (p < 0.001, p < 0.05 and p < 0.001; respectively). Significance: We concluded that the blockade of the renin-angiotensin system with ramipril reduced early markers of diabetic nephropathy, a phenomenon that cannot be specifically related to decreased BP levels. (C) 2013 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 12/04831-1 - Novos moduladores do controle glicêmico e do desenvolvimento de complicações crônicas no Diabetes mellitus: perspectivas preventivas e terapêuticas
Beneficiário:Ubiratan Fabres Machado
Linha de fomento: Auxílio à Pesquisa - Temático