Hippocampal NMDA receptor blockade impairs CREB phosphorylation in amygdala after contextual fear conditioning

Autor(es)

de Oliveira Coelho, Cesar Augusto ^[1] ; Ferreira, Tatiana Lima ; Kramer Soares, Juliana Carlota ^[1] ; Menezes Oliveira, Maria Gabriela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psicobiol, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HIPPOCAMPUS; v. 23, n. 7, p. 545-551, JUL 2013.
• Citações Web of Science: 7

Resumo

In contextual fear conditioning (CFC), hippocampus is thought to process environmental stimuli into a configural representation of the context and send it to amygdala nuclei, which current evidences point to be the site of CS-US association and fear memory storage. If it is true, hippocampus should influence learning-induced plasticity in the amygdala nuclei after CFC acquisition. To test this, we infused wistar rats with saline or AP5, a NMDA receptor antagonist, in the dorsal hippocampus just before a CFC session, in which they were conditioned to a single shock, exposed to the context with no shocks or received an immediate shock. The rats were perfused, their brains harvested and immunohistochemically stained for cAMP element binding protein (CREB) phosphorylation ratio (pCREB/CREB) in lateral (LA), basal (B) and central (CeA) amygdala nuclei. CFC showed a learning-specific increase in pCREB ratio in B and CeA, in conditioned-saline rats compared to context and immediate shocked ones. Further, conditioned rats that received AP5 showed a decrease in pCREB ratio in LA, B and CeA. Our results support the current ideas that the role of hippocampus in contextual fear conditioning occurs by sending contextual information to amygdala to serve as conditioned stimulus. (c) 2013 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 10/02509-0 - Influência do hipocampo na expressão de CREB e pCREB nos núcleos da amígdala após condicionamento de medo ao contexto
• Beneficiário: Cesar Augusto de Oliveira Coelho
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/16295-1 - Influência do hipocampo na expressão de CREB e pCREB nos núcleos da amígdala após condicionamento de medo ao contexto
• Beneficiário: Maria Gabriela Menezes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular