New staining technique for fungal-infected plant tissues

Autor(es)

Rodrigues Marques, Joao Paulo ^[1] ; Misaki Soares, Marli Kasue ^[1] ; Appezzato-Da-Gloria, Beatriz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Biol Sci, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TURKISH JOURNAL OF BOTANY; v. 37, n. 4, p. 784-787, 2013.
• Citações Web of Science: 7

Resumo

The use of histological techniques, combined with the use of dyes, facilitates the visualisation of fungi present in tissues, allowing the details of plant pathogen interactions in many different pathosystems to be revealed. The purpose of this study is to present anew double-staining method that uses cotton blue and safranin to identify fungal structures in plant tissues. With this staining method, the fungal hyphae stain blue and plant cell walls stain orange. The new method was tested on 2 pathosystems. The technique distinguishes between fungal and plant cell walls, giving it great practical utility. This is a staining technique that can be used routinely in laboratories and facilitates the creation of permanent slides. (AU)

• Processo FAPESP: 09/00425-6 - Podridão floral dos citros: histopatologia de Colletotrichum acutatum
• Beneficiário: João Paulo Rodrigues Marques
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/54176-4 - Epidemiologia molecular e manejo da podridão floral dos citros em áreas de expansão da cultura no estado de São Paulo
• Beneficiário: Lilian Amorim
• Modalidade de apoio: Auxílio à Pesquisa - Temático