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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

New staining technique for fungal-infected plant tissues

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Author(s):
Rodrigues Marques, Joao Paulo [1] ; Misaki Soares, Marli Kasue [1] ; Appezzato-Da-Gloria, Beatriz [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Biol Sci, Piracicaba - Brazil
Total Affiliations: 1
Document type: Journal article
Source: TURKISH JOURNAL OF BOTANY; v. 37, n. 4, p. 784-787, 2013.
Web of Science Citations: 7
Abstract

The use of histological techniques, combined with the use of dyes, facilitates the visualisation of fungi present in tissues, allowing the details of plant pathogen interactions in many different pathosystems to be revealed. The purpose of this study is to present anew double-staining method that uses cotton blue and safranin to identify fungal structures in plant tissues. With this staining method, the fungal hyphae stain blue and plant cell walls stain orange. The new method was tested on 2 pathosystems. The technique distinguishes between fungal and plant cell walls, giving it great practical utility. This is a staining technique that can be used routinely in laboratories and facilitates the creation of permanent slides. (AU)

FAPESP's process: 09/00425-6 - Postbloom fruit drop of citrus: histopathology of Colletotrichum acutatum
Grantee:João Paulo Rodrigues Marques
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 08/54176-4 - Molecular epidemiology and management of postbloom fruit drop of citrus in new planting areas in São Paulo State
Grantee:Lilian Amorim
Support Opportunities: Research Projects - Thematic Grants