| Texto completo | |
| Autor(es): |
Manetti, M. E.
[1]
;
Rossi, M.
[1]
;
Nakabashi, M.
[1]
;
Grandbastien, M. A.
[2]
;
Van Sluys, Marie Anne
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, GaTE Lab, Dept Bot, Inst Biociencias, IBUSP, BR-05508090 Sao Paulo - Brazil
[2] INRA, Biol Cellulaire Lab, Inst Jean Pierre Bourgin, F-78026 Versailles - France
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Molecular Genetics and Genomics; v. 281, n. 3, p. 261-271, Mar. 2009. |
| Área do conhecimento: | Ciências Biológicas - Genética |
| Citações Web of Science: | 6 |
| Assunto(s): | Elementos de DNA transponíveis Retroelementos Plantas Solanum Solanaceae |
| Resumo | |
Eukaryotic genome expansion/retraction caused by LTR-retrotransposon activity is dependent on the expression of full length copies to trigger efficient transposition and recombination-driven events. The Tnt1 family of retrotransposons has served as a model to evaluate the diversity among closely related elements within Solanaceae species and found that members of the family vary mainly in their U3 region of the long terminal repeats (LTRs). Recovery of a full length genomic copy of Retrosol was performed through a PCR-based approach from wild potato, Solanum oplocense. Further characterization focusing on both LTR sequences of the amplified copy allowed estimating an approximate insertion time at 2 million years ago thus supporting the occurrence of transposition cycles after genus divergence. Copy number of Tnt1-like elements in Solanum species were determined through genomic quantitative PCR whereby results sustain that Retrosol in Solanum species is a low copy number retrotransposon (1–4 copies) while Retrolyc1 has an intermediate copy number (38 copies) in S. peruvianum. Comparative analysis of retrotransposon content revealed no correlation between genome size or ploidy level and Retrosol copy number. The tetraploid cultivated potato with a cellular genome size of 1,715 Mbp harbours similar copy number per monoploid genome than other diploid Solanum species (613–884 Mbp). Conversely, S. peruvianum genome (1,125 Mbp) has a higher copy number. These results point towards a lineage specific dynamic flux regarding the history of amplification/activity of Tnt1-like elements in the genome of Solanum species. (AU) | |
| Processo FAPESP: | 08/55646-4 - A família de retrotransposons TNT1 de Solanaceae: evolução, diversificação e função |
| Beneficiário: | Marie-Anne Van Sluys |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |