| Texto completo | |
| Autor(es): |
Magdalena Laurito
[1]
;
Tatiane MP de Oliveira
[2]
;
Walter Ricardo Almiron
[3]
;
Maria Anice Mureb Sallum
[4]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Universidad Nacional de Cordoba. Facultad de Ciencias Exactas, Fisicas y Naturales. Centro de Investigaciones Entomologicas de Cordoba - Argentina
[2] Universidade de Sao Paulo. Faculdade de Saude Publica. Departamento de Epidemiologia - Brasil
[3] Universidad Nacional de Cordoba. Facultad de Ciencias Exactas, Fisicas y Naturales. Centro de Investigaciones Entomologicas de Cordoba - Argentina
[4] Universidade de Sao Paulo. Faculdade de Saude Publica. Departamento de Epidemiologia - Brasil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Memórias do Instituto Oswaldo Cruz; v. 108, p. 110-122, 2013-00-00. |
| Resumo | |
Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex ( Culex ) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus , Cx. chidesteri , Cx. dolosus , Cx. lygrus and Cx. saltanensis ) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus . Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex ( Cux. ) species. (AU) | |
| Processo FAPESP: | 11/20397-7 - Taxonomia morfológica e molecular e filogenia do subgênero Nyssorhynchus do gênero Anopheles (Diptera: Culicidae), com ênfase especial em Anopheles darlingi da Mata Atlântica |
| Beneficiário: | Maria Anice Mureb Sallum |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |