Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

COI barcode versus morphological identification of Culex ( Culex ) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil

Full text
Author(s):
Magdalena Laurito [1] ; Tatiane MP de Oliveira [2] ; Walter Ricardo Almiron [3] ; Maria Anice Mureb Sallum [4]
Total Authors: 4
Affiliation:
[1] Universidad Nacional de Cordoba. Facultad de Ciencias Exactas, Fisicas y Naturales. Centro de Investigaciones Entomologicas de Cordoba - Argentina
[2] Universidade de Sao Paulo. Faculdade de Saude Publica. Departamento de Epidemiologia - Brasil
[3] Universidad Nacional de Cordoba. Facultad de Ciencias Exactas, Fisicas y Naturales. Centro de Investigaciones Entomologicas de Cordoba - Argentina
[4] Universidade de Sao Paulo. Faculdade de Saude Publica. Departamento de Epidemiologia - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 108, p. 110-122, 2013-00-00.
Abstract

Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex ( Culex ) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus , Cx. chidesteri , Cx. dolosus , Cx. lygrus and Cx. saltanensis ) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus . Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex ( Cux. ) species. (AU)

FAPESP's process: 11/20397-7 - Morphological and molecular taxonomy and phylogeny of Nyssorhynchus subgenus of Anopheles (Diptera: Culicidae), with special emphasis on Anopheles darlingi from Mata Atlântica
Grantee:Maria Anice Mureb Sallum
Support Opportunities: Regular Research Grants