Transcriptional Activity, Chromosomal Distribution and Expression Effects of Transposable Elements in Coffea Genomes

Autor(es):Mostrar menos -

Lopes, Fabricio R. ^[1] ; Jjingo, Daudi ^[2] ; da Silva, Carlos R. M. ^[3] ; Andrade, Alan C. ^[4] ; Marraccini, Pierre ^{[4, 5]} ; Teixeira, Joao B. ^[4] ; Carazzolle, Marcelo F. ^[6] ; Pereira, Goncalo A. G. ^[6] ; Pereira, Luiz Filipe P. ^[7] ; Vanzela, Andre L. L. ^[3] ; Wang, Lu ^[2] ; King Jordan, I. ^{[2, 8]} ; Carareto, Claudia M. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Univ Estadual Paulista Julio de Mesquita Filho, Dept Biol, Sao Jose do Rio Preto - Brazil

^[2] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 - USA

^[3] Univ Estadual Londrina, Dept Biol Geral, Londrina - Brazil

^[4] Empresa Brasileira Pesquisa Agr Recursos Genet &, Brasilia, DF - Brazil

^[5] Ctr Cooperat Int Rech Agron Dev, UMR AGAP, Montpellier - France

^[6] Univ Estadual Campinas, Dept Genet Evolucao & Bioagentes, Campinas, SP - Brazil

^[7] Empresa Brasileira Pesquisa Agr Cafe, Brasilia, DF - Brazil

^[8] PanAmer Bioinformat Inst, Santa Marta, Magdalena - Colombia

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 8, n. 11 NOV 11 2013.
• Citações Web of Science: 12

Resumo

Plant genomes are massively invaded by transposable elements (TEs), many of which are located near host genes and can thus impact gene expression. In flowering plants, TE expression can be activated (de-repressed) under certain stressful conditions, both biotic and abiotic, as well as by genome stress caused by hybridization. In this study, we examined the effects of these stress agents on TE expression in two diploid species of coffee, Coffea canephora and C. eugenioides, and their allotetraploid hybrid C. arabica. We also explored the relationship of TE repression mechanisms to host gene regulation via the effects of exonized TE sequences. Similar to what has been seen for other plants, overall TE expression levels are low in Coffea plant cultivars, consistent with the existence of effective TE repression mechanisms. TE expression patterns are highly dynamic across the species and conditions assayed here are unrelated to their classification at the level of TE class or family. In contrast to previous results, cell culture conditions per se do not lead to the de-repression of TE expression in C. arabica. Results obtained here indicate that differing plant drought stress levels relate strongly to TE repression mechanisms. TEs tend to be expressed at significantly higher levels in non-irrigated samples for the drought tolerant cultivars but in drought sensitive cultivars the opposite pattern was shown with irrigated samples showing significantly higher TE expression. Thus, TE genome repression mechanisms may be finely tuned to the ideal growth and/or regulatory conditions of the specific plant cultivars in which they are active. Analysis of TE expression levels in cell culture conditions underscored the importance of nonsense-mediated mRNA decay (NMD) pathways in the repression of Coffea TEs. These same NMD mechanisms can also regulate plant host gene expression via the repression of genes that bear exonized TE sequences. (AU)

• Processo FAPESP: 07/55985-0 - Análise da ocorrência de elementos de transposição em ESTs de três espécies de Coffea geradas pelo Projeto Genoma Café
• Beneficiário: Fabrício Ramon Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 08/05894-1 - Análise da expressão de genes portadores de cassetes de elementos transponíveis e elementos ativos em espécies diplóides (C. canephora, C. eugenoides, C. racemosa) e poliplóide (C. arabica) de Coffea
• Beneficiário: Claudia Marcia Aparecida Carareto
• Modalidade de apoio: Auxílio à Pesquisa - Regular