| Texto completo | |
| Autor(es): |
Pires Goncalves, Leticia Christina
[1]
;
Da Silva, Sandra Maria
[2]
;
DeRose, Paul C.
[2]
;
Ando, Romulo Augusto
[3]
;
Bastos, Erick Leite
[3]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] NIST, Biosyst & Biomat Div, Chem Sci Technol Lab, Gaithersburg, MD 20899 - USA
[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 8, n. 9 SEP 3 2013. |
| Citações Web of Science: | 17 |
| Resumo | |
In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent orange 1: 1 complex with a stability constant of 1.4x10(5) L mol(-1). The addition of calcium dipicolinate, largely found in bacterial spores, changes the color of the aqueous solution of {[}Eu(Bn)(+)] from orange to magenta. The limit of detection (LOD) of calcium dipicolinate is around 2.0x10(-6) mol L-1 and the LOD determined for both spores, B. cereus and B. anthracis, is (1.1 +/- 0.3)x10(6) spores mL(-1). This simple, green, fast and low cost colorimetric assay was selective for calcium dipicolinate when compared to several analogous compounds. The importance of this work relies on the potential use of betalains, raw natural pigments, as colorimetric sensors for biological applications. (AU) | |
| Processo FAPESP: | 11/23036-5 - Pigmentos betalâmicos de flores: fluorescência e capacidade antirradicalar |
| Beneficiário: | Erick Leite Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 07/00684-6 - Sintese e aplicacao de derivados de pimentos de flores. |
| Beneficiário: | Erick Leite Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 07/59407-1 - Betalaínas semissintéticas como antioxidantes e pigmentos biocompatíveis |
| Beneficiário: | Leticia Christina Pires Gonçalves |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |