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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Beetroot-Pigment-Derived Colorimetric Sensor for Detection of Calcium Dipicolinate in Bacterial Spores

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Pires Goncalves, Leticia Christina [1] ; Da Silva, Sandra Maria [2] ; DeRose, Paul C. [2] ; Ando, Romulo Augusto [3] ; Bastos, Erick Leite [3]
Total Authors: 5
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] NIST, Biosyst & Biomat Div, Chem Sci Technol Lab, Gaithersburg, MD 20899 - USA
[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 8, n. 9 SEP 3 2013.
Web of Science Citations: 17

In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent orange 1: 1 complex with a stability constant of 1.4x10(5) L mol(-1). The addition of calcium dipicolinate, largely found in bacterial spores, changes the color of the aqueous solution of {[}Eu(Bn)(+)] from orange to magenta. The limit of detection (LOD) of calcium dipicolinate is around 2.0x10(-6) mol L-1 and the LOD determined for both spores, B. cereus and B. anthracis, is (1.1 +/- 0.3)x10(6) spores mL(-1). This simple, green, fast and low cost colorimetric assay was selective for calcium dipicolinate when compared to several analogous compounds. The importance of this work relies on the potential use of betalains, raw natural pigments, as colorimetric sensors for biological applications. (AU)

FAPESP's process: 11/23036-5 - Floral betalamic pigments: fluorescence and antiradical capacity
Grantee:Erick Leite Bastos
Support type: Regular Research Grants
FAPESP's process: 07/00684-6 - Synthesis and application of modified flower pigments
Grantee:Erick Leite Bastos
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 07/59407-1 - Semi-synthetic betalains as biocompatible antioxidants and pigments
Grantee:Leticia Christina Pires Gonçalves
Support type: Scholarships in Brazil - Doctorate (Direct)