Microemulsion extrusion technique: a new method to produce lipid nanoparticles

Autor(es)

de Jesus, Marcelo Bispo ^{[1, 2, 3]} ; Radaic, Allan ^[2] ; Zuhorn, Inge S. ^[3] ; de Paula, Eneida ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem, BR-13083862 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem, BR-13083970 Campinas, SP - Brazil

^[3] Univ Groningen, Univ Med Ctr Groningen, Dept Membrane Cell Biol, NL-9713 AV Groningen - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NANOPARTICLE RESEARCH; v. 15, n. 10 SEP 3 2013.
• Citações Web of Science: 7

Resumo

Solid lipid nanoparticles (SLN) and nano-structured lipid carriers (NLC) have been intensively investigated for different applications, including their use as drug and gene delivery systems. Different techniques have been employed to produce lipid nanoparticles, of which high pressure homogenization is the standard technique that is adopted nowadays. Although this method has a high efficiency, does not require the use of organic solvents, and allows large-scale production, some limitations impede its application at laboratory scale: the equipment is expensive, there is a need of huge amounts of surfactants and co-surfactants during the preparation, and the operating conditions are energy intensive. Here, we present the microemulsion extrusion technique as an alternative method to prepare lipid nanoparticles. The parameters to produce lipid nanoparticles using microemulsion extrusion were established, and the lipid particles produced (SLN, NLC, and liposomes) were characterized with regard to size (from 130 to 190 nm), zeta potential, and drug (mitoxantrone) and gene (pDNA) delivery properties. In addition, the particles' in vitro co-delivery capacity (to carry mitoxantrone plus pDNA encoding the phosphatase and tensin homologue, PTEN) was tested in normal (BALB3T3 fibroblast) and cancer (PC3 prostate and MCF-7 breast) cell lines. The results show that the microemulsion extrusion technique is fast, inexpensive, reproducible, free of organic solvents, and suitable for small volume preparations of lipid nanoparticles. Its application is particularly interesting when using rare and/or costly drugs or ingredients (e. g., cationic lipids for gene delivery or labeled lipids for nanoparticle tracking/diagnosis). (AU)

• Processo FAPESP: 12/01038-9 - Nanopartículas lipídicas como carreadoras de fármacos e genes: desenvolvimento, tracking do processamento intracelular e atividade biológica em células de câncer
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/13110-3 - Nanopartículas lipídicas sólidas (NLS) para o transporte de RNAi: Estudo do papel biológico da proteína quinase regulada por sinais extracelulares (ERK) em células de câncer de próstata.
• Beneficiário: Allan Radaic
• Modalidade de apoio: Bolsas no Brasil - Mestrado