Formaldehyde inhalation reduces respiratory mechanics in a rat model with allergic lung inflammation by altering the nitric oxide/cyclooxygenase-derived products relationship

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Lino-dos-Santos-Franco, Adriana ^{[1, 2]} ; Gimenes-Junior, Joao Antonio ^[1] ; Ligeiro-de-Oliveira, Ana Paula ^[3] ; Breithaupt-Faloppa, Ana Cristina ^[4] ; Acceturi, Beatriz Golega ^[1] ; Vitoretti, Luana Beatriz ^[1] ; Machado, Isabel Daufenback ^[2] ; Oliveira-Filho, Ricardo Martins ^[1] ; Poliselli Farsky, Sandra Helena ^[2] ; Moriya, Henrique Takachi ^[5] ; Tavares-de-Lima, Wothan ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508900 Sao Paulo - Brazil

^[3] Nove Julho Univ, Post Grad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Sci, Hosp Clin, BR-05508900 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Engn, Dept Telecommun & Control Engn, Biomed Engn Lab, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Chemical Toxicology; v. 59, p. 731-738, SEP 2013.
• Citações Web of Science: 4

Resumo

Bronchial hyperresponsiveness is a hallmark of asthma and many factors modulate bronchoconstriction episodes. A potential correlation of formaldehyde (FA) inhalation and asthma has been observed; however, the exact role of FA remains controversial. We investigated the effects of FA inhalation on Ovalbumin (OVA) sensitisation using a parameter of respiratory mechanics. The involvement of nitric oxide (NO) and cyclooxygenase-derived products were also evaluated. The rats were submitted, or not, to FA inhalation (1%, 90 min/day, 3 days) and were OVA-sensitised and challenged 14 days later. Our data showed that previous FA exposure in allergic rats reduced bronchial responsiveness, respiratory resistance (Rrs) and elastance (Ers) to methacholine. FA exposure in allergic rats also increased the iNOS gene expression and reduced COX-1. L-NAME treatment exacerbated the bronchial hyporesponsiveness and did not modify the Ers and Rrs, while Indomethacin partially reversed all of the parameters studied. The L-NAME and Indomethacin treatments reduced leukotriene B-4 levels while they increased thromboxane B-2 and prostaglandin E-2. In conclusion, FA exposure prior to OVA sensitisation reduces the respiratory mechanics and the interaction of NO and PGE(2) may be representing a compensatory mechanism in order to protect the lung from bronchoconstriction effects. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/51886-3 - Neuroimunomodulação: fármacos, estresse e citocinas nas relações entre os sistemas nervoso, endócrino e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático