Acute exercise suppresses hypothalamic PTP1B protein level and improves insulin and leptin signaling in obese rats

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Chiarreotto-Ropelle, Eloize C. ^[1] ; Pauli, Luciana S. S. ^[1] ; Katashima, Carlos K. ^[2] ; Pimentel, Gustavo D. ^[2] ; Picardi, Paty K. ^[2] ; Silva, Vagner R. R. ^[1] ; de Souza, Claudio T. ^[3] ; Prada, Patricia O. ^[1] ; Cintra, Dennys E. ^[1] ; Carvalheira, Jose B. C. ^[2] ; Ropelle, Eduardo R. ^{[2, 1]} ; Pauli, Jose R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Sch Appl Sci, Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, Campinas, SP - Brazil

^[3] Univ Southern Santa Catarina, Criciuma, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM; v. 305, n. 5, p. E649-E659, SEP 2013.
• Citações Web of Science: 24

Resumo

Hypothalamic inflammation is associated with insulin and leptin resistance, hyperphagia, and obesity. In this scenario, hypothalamic protein tyrosine phosphatase 1B (PTP1B) has emerged as the key phosphatase induced by inflammation that is responsible for the central insulin and leptin resistance. Here, we demonstrated that acute exercise reduced inflammation and PTP1B protein level/activity in the hypothalamus of obese rodents. Exercise disrupted the interaction between PTP1B with proteins involved in the early steps of insulin (IR beta and IRS-1) and leptin (JAK2) signaling, increased the tyrosine phosphorylation of these molecules, and restored the anorexigenic effects of insulin and leptin in obese rats. Interestingly, the anti-inflammatory action and the reduction of PTP1B activity mediated by exercise occurred in an interleukin-6 (IL-6)-dependent manner because exercise failed to reduce inflammation and PTP1B protein level after the disruption of hypothalamic-specific IL-6 action in obese rats. Conversely, intracerebroventricular administration of recombinant IL-6 reproduced the effects of exercise, improving hypothalamic insulin and leptin action by reducing the inflammatory signaling and PTP1B activity in obese rats at rest. Taken together, our study reports that physical exercise restores insulin and leptin signaling, at least in part, by reducing hypothalamic PTP1B protein level through the central anti-inflammatory response. (AU)

• Processo FAPESP: 11/14727-4 - Caracterização da atividade da PTP1B em hipotálamo de roedores obesos submetidos ao exercício físico.
• Beneficiário: Eloize Cristina Chiarreotto Ropelle
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/13779-0 - Efeitos do exercício físico sobre a associação MKP-3/FoxO1 em tecido hepático de camundongos obesos e diabéticos
• Beneficiário: Luciana Santos Souza Pauli
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/12091-2 - Efeitos do treinamento físico sobre o processo inflamatório e apoptótico em neurônios hipotalâmicos de controle da fome em camundongos obesos induzidos por dieta hiperlipídica
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular