Generation of reactive oxygen species by leukocytes of Prochilodus lineatus

Autor(es)

de Faria, Marcos Tucunduva ^[1] ; Cury-Boaventura, Maria Fernanda ^[2] ; Lopes, Lucia Rossetti ^[3] ; Machado Cunha da Silva, Jose Roberto ^{[4, 5]}

Afiliação do(s) autor(es)

^[1] EMBRAPA, Brazilian Agr Res Corp, BR-66095130 Belem, Para - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Cellular & Dev Biol, Inst Biomed Sci, Sao Paulo - Brazil

^[5] Univ Sao Paulo, CEBIMar, Marine Biol Ctr, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FISH PHYSIOLOGY AND BIOCHEMISTRY; v. 40, n. 2, p. 445-455, APR 2014.
• Citações Web of Science: 4

Resumo

Prochilodus lineatus (curimbata), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O-2 (-), using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O-2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47(phox) and p67(phox)). Thus, catalase enhanced the presence of p47(phox). Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. (AU)

• Processo FAPESP: 01/08205-3 - Mecanismos de resistência natural no Curimbatá (Prochilodus lineatus, Valenciennes, 1836)
• Beneficiário: José Roberto Machado Cunha da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular