Bone marrow mesenchymal stem cells stimulated by bFGF up-regulated protein expression in comparison with periodontal fibroblasts in vitro

Autor(es)

Colenci, Renato ^[1] ; da Silva Assuncao, Luciana Reichert ^[2] ; Mogami Bomfim, Suely Regina ^[3] ; Golim, Marjorie de Assis ^[4] ; Deffune, Elenice ^[5] ; Penha Oliveira, Sandra Helena ^[6]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, DDS, Sch Dent, Sao Paulo - Brazil

^[2] Univ Fed Parana, UFPR, Sch Dent, Dept Stornatol, BR-80060000 Curitiba, Parana - Brazil

^[3] UNESP Univ Estadual Paulista, Sch Vet Med, Dept Clin Surg & Anim Reprod, Sao Paulo - Brazil

^[4] UNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Flow Cytometry, Sao Paulo - Brazil

^[5] UNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Cellular Engn, Sao Paulo - Brazil

^[6] UNESP Univ Estadual Paulista, Dept Basic Sci, Sch Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 59, n. 3, p. 268-276, MAR 2014.
• Citações Web of Science: 5

Resumo

Objective: The aim of this study was to evaluate, in vitro, the role of bFGF in the proliferation and expression of collagen type I and fibronectin of dog bone marrow mesenchymal stem cells (dBMMSCs) in comparison with the expression of the same proteins in dog periodontal fibroblasts (dPLFs). Design: dBMMSCs from the iliac crest were cultivated in Dulbecco's Modified Eagle's Medium (DMEM). Flow cytometry analysis (FCA) was used to characterize dBMMSC. Cells were stimulated with bFGF (1, 5 and 10 ng/mL) after 24 and 48 h. Real time RT-PCR was performed to verify collagen type I and fibronectin expressions. MTT assay was used to confirm cellular proliferation. Statistical analyses were performed (ANOVA and Kruskal Wallis tests; p < 0.05). Results: FCA showed 55.98% of CD34+ and 32.67% of CD90+ after bone marrow aspiration; 3.33% of CD34+ and 33.0% of CD90+ before P1. After P2, 10.54% of dBMMSCs expressed CD90, whereas after P3, this number decreased to 1.58%. dPLFs presented 4.04% of CD90+ and 1.05% of CD34+ after P3. MU evaluation showed increase in dBMSC proliferation with 5 ng/mL bFGF-stimulus after 24-h. Both collagen land fibronectin expression were very similar between the two cells groups after 24-h stimulation with 1 ng/mL bFGF concentration. Fibronectin and collagen I expressions were higher after 24-h stimulation with 5 ng/mL bFGF. Conclusion: dBMMSCs (1 ng/mL-bFGF stimulus after 24 h) are very similar to dPLFs as regards morphological and immunostaining characteristics, and collagen and/or fibronectin production. The dBMMSCs presented the highest protein expression rates with 5 ng/mL-bFGF stimulus after 24-h. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/59420-5 - Efeito das células-tronco originárias da medula óssea na regeneração do ligamento periodontal após reimplante dentário: estudo em cães
• Beneficiário: Sandra Helena Penha de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular