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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bone marrow mesenchymal stem cells stimulated by bFGF up-regulated protein expression in comparison with periodontal fibroblasts in vitro

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Author(s):
Colenci, Renato [1] ; da Silva Assuncao, Luciana Reichert [2] ; Mogami Bomfim, Suely Regina [3] ; Golim, Marjorie de Assis [4] ; Deffune, Elenice [5] ; Penha Oliveira, Sandra Helena [6]
Total Authors: 6
Affiliation:
[1] UNESP Univ Estadual Paulista, DDS, Sch Dent, Sao Paulo - Brazil
[2] Univ Fed Parana, UFPR, Sch Dent, Dept Stornatol, BR-80060000 Curitiba, Parana - Brazil
[3] UNESP Univ Estadual Paulista, Sch Vet Med, Dept Clin Surg & Anim Reprod, Sao Paulo - Brazil
[4] UNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Flow Cytometry, Sao Paulo - Brazil
[5] UNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Cellular Engn, Sao Paulo - Brazil
[6] UNESP Univ Estadual Paulista, Dept Basic Sci, Sch Dent, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 59, n. 3, p. 268-276, MAR 2014.
Web of Science Citations: 5
Abstract

Objective: The aim of this study was to evaluate, in vitro, the role of bFGF in the proliferation and expression of collagen type I and fibronectin of dog bone marrow mesenchymal stem cells (dBMMSCs) in comparison with the expression of the same proteins in dog periodontal fibroblasts (dPLFs). Design: dBMMSCs from the iliac crest were cultivated in Dulbecco's Modified Eagle's Medium (DMEM). Flow cytometry analysis (FCA) was used to characterize dBMMSC. Cells were stimulated with bFGF (1, 5 and 10 ng/mL) after 24 and 48 h. Real time RT-PCR was performed to verify collagen type I and fibronectin expressions. MTT assay was used to confirm cellular proliferation. Statistical analyses were performed (ANOVA and Kruskal Wallis tests; p < 0.05). Results: FCA showed 55.98% of CD34+ and 32.67% of CD90+ after bone marrow aspiration; 3.33% of CD34+ and 33.0% of CD90+ before P1. After P2, 10.54% of dBMMSCs expressed CD90, whereas after P3, this number decreased to 1.58%. dPLFs presented 4.04% of CD90+ and 1.05% of CD34+ after P3. MU evaluation showed increase in dBMSC proliferation with 5 ng/mL bFGF-stimulus after 24-h. Both collagen land fibronectin expression were very similar between the two cells groups after 24-h stimulation with 1 ng/mL bFGF concentration. Fibronectin and collagen I expressions were higher after 24-h stimulation with 5 ng/mL bFGF. Conclusion: dBMMSCs (1 ng/mL-bFGF stimulus after 24 h) are very similar to dPLFs as regards morphological and immunostaining characteristics, and collagen and/or fibronectin production. The dBMMSCs presented the highest protein expression rates with 5 ng/mL-bFGF stimulus after 24-h. (C) 2013 Elsevier Ltd. All rights reserved. (AU)