Evaluation of Intestinal Permeability of Vicenin-2 and Lychnopholic Acid from Lychnophora salicifolia (Brazilian Arnicao) Using Caco-2 Cells

Autor(es)

Gouvea, Dayana Rubio ^[1] ; Bello Ribeiro, Arthur de Barros ^[1] ; Thormann, Ursula ^[2] ; Lopes, Norberto Peporine ^[1] ; Butterweck, Veronika ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS Nucleo Pesquisa Prod Nat & Sintet, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Appl Sci Northwestern Switzerland, Inst Pharma Technol, Sch Life Sci, CH-4132 Muttenz - Switzerland

• Tipo de documento: Artigo Científico
• Fonte: Journal of Natural Products; v. 77, n. 3, p. 464-471, MAR 2014.
• Citações Web of Science: 5

Resumo

Lychnophora salicifolia, commonly known as ``arnicao{''}, is used as an anti-inflammatory agent and as a flavoring agent in the Brazilian traditional spirit ``cachaca{''}. In this work, the permeation process of vicenin-2 (1) and lychnopholic acid (2) (major secondary metabolites from the hydroalcoholic extract) was investigated using Caco-2 cells. For this investigation, a new HPLC-DAD method was developed and validated for the quantification step. It was observed that 2 crosses the Caco-2 cell monolayer by passive diffusion. On the other hand, 1 was not transported, suggesting no absorption and no efflux of this compound in Caco-2 cells. (AU)

• Processo FAPESP: 10/09137-0 - "Estudos in vitro e in vivo do metabolismo dos compostos majoritários presentes no extrato das folhas de Lychnophora salicifolia Mart. (Asteraceae: Vernonieae)"
• Beneficiário: Dayana Rubio Gouvea
• Modalidade de apoio: Bolsas no Brasil - Doutorado