Evaluation of Intestinal Permeability of Vicenin-2 and Lychnopholic Acid from Lychnophora salicifolia (Brazilian Arnicao) Using Caco-2 Cells

Author(s)

Gouvea, Dayana Rubio ^[1] ; Bello Ribeiro, Arthur de Barros ^[1] ; Thormann, Ursula ^[2] ; Lopes, Norberto Peporine ^[1] ; Butterweck, Veronika ^[2]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS Nucleo Pesquisa Prod Nat & Sintet, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Appl Sci Northwestern Switzerland, Inst Pharma Technol, Sch Life Sci, CH-4132 Muttenz - Switzerland

• Document type: Journal article
• Source: Journal of Natural Products; v. 77, n. 3, p. 464-471, MAR 2014.
• Web of Science Citations: 5

Abstract

Lychnophora salicifolia, commonly known as ``arnicao{''}, is used as an anti-inflammatory agent and as a flavoring agent in the Brazilian traditional spirit ``cachaca{''}. In this work, the permeation process of vicenin-2 (1) and lychnopholic acid (2) (major secondary metabolites from the hydroalcoholic extract) was investigated using Caco-2 cells. For this investigation, a new HPLC-DAD method was developed and validated for the quantification step. It was observed that 2 crosses the Caco-2 cell monolayer by passive diffusion. On the other hand, 1 was not transported, suggesting no absorption and no efflux of this compound in Caco-2 cells. (AU)

• FAPESP's process: 10/09137-0 - "In vitro and in vivo metabolism of the major secondary metabolites present in the extract of Lychnophora salicifolia Mart. (Asteraceae: Vernonieae) leaves"
• Grantee: Dayana Rubio Gouvea
• Support Opportunities: Scholarships in Brazil - Doctorate