The increase of antimicrobial resistance is a major health problem. There is a current interest on the development of new drugs that could act against infeccious agents, and on the optimization of their administration to avoid such cases. A possible strategy to optimize the drug administration is to study their biopharmaceutical characteristcs, such as the determination of sistemic and local bioavailability, speed of permeability through the tissue and interactions with tranporters. In vitro models have been employed for these purposes, and an FDA guidance supports the use of cell culture models for the determination of permeability of new drugs. Since most drugs are developed for oral delivery, the intestinal Caco-2 cell line is probably the most widely used for intestinal permeability determination and drug screening. In this study, we will use cell culture models to investigate the biopharmaceutical characteristics of gallic acid in its isolated form, in complex matrix as a plant extract and in modified release formulation. Gallic acid is a natural bioactive that has demonstrated a promising antifungal activity against Candida albicans. We have selected in vitro models of intestinal (Caco- 2) and oral cells (RHOE, Reconstituted Human Oral Epithelium), since these tissues are initial sites of infection by Candida albicans. Both cells will be infected with Candida albicans in order to evaluate the antifungal activity of gallic acid as a function of time. Once developed, these models will be used to determine the biopharmaceutical characteristics and/or the bioactivity of other compounds and bioactive products which have been isolated and studied in our laboratory.
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