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DEPLOYMENT OF ASSAYS FOR EVALUATION OF BIOPHARMACEUTIC CHARACTERISTICS AND BIOACTIVITY OF COMPOUNDS AND BIOACTIVE PRODUCTS USING IN VITRO MODELS OF INVASIVE CANDIDIASIS

Grant number: 14/09666-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2014
Effective date (End): December 31, 2017
Field of knowledge:Health Sciences - Pharmacy - Medicines Analysis and Control
Principal researcher:Marcos José Salvador
Grantee:Aline Vidal Lacerda Gontijo
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The increase of antimicrobial resistance is a major health problem. There is a current interest on the development of new drugs that could act against infeccious agents, and on the optimization of their administration to avoid such cases. A possible strategy to optimize the drug administration is to study their biopharmaceutical characteristcs, such as the determination of sistemic and local bioavailability, speed of permeability through the tissue and interactions with tranporters. In vitro models have been employed for these purposes, and an FDA guidance supports the use of cell culture models for the determination of permeability of new drugs. Since most drugs are developed for oral delivery, the intestinal Caco-2 cell line is probably the most widely used for intestinal permeability determination and drug screening. In this study, we will use cell culture models to investigate the biopharmaceutical characteristics of gallic acid in its isolated form, in complex matrix as a plant extract and in modified release formulation. Gallic acid is a natural bioactive that has demonstrated a promising antifungal activity against Candida albicans. We have selected in vitro models of intestinal (Caco- 2) and oral cells (RHOE, Reconstituted Human Oral Epithelium), since these tissues are initial sites of infection by Candida albicans. Both cells will be infected with Candida albicans in order to evaluate the antifungal activity of gallic acid as a function of time. Once developed, these models will be used to determine the biopharmaceutical characteristics and/or the bioactivity of other compounds and bioactive products which have been isolated and studied in our laboratory.

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GONTIJO, ALINE V. L.; SAMPAIO, ALINE DA G.; KOGA-ITO, CRISTIANE Y.; SALVADOR, MARCOS J. Biopharmaceutical and antifungal properties of ellagic acid-cyclodextrin using an in vitro model of invasive candidiasis. FUTURE MICROBIOLOGY, v. 14, n. 11, p. 957-967, JUL 2019. Web of Science Citations: 0.
TEODORO, GUILHERME R.; GONTIJO, ALINE V. L.; SALVADOR, MARCOS J.; TANAKA, MARCIA H.; BRIGHENTI, FERNANDA L.; DELBEM, ALBERTO C. B.; DELBEM, ADINA C. B.; KOGA-ITO, CRISTIANE Y. Effects of Acetone Fraction From Buchenavia tomentosa Aqueous Extract and Gallic Acid on Candida albicans Biofilms and Virulence Factors. FRONTIERS IN MICROBIOLOGY, v. 9, APR 5 2018. Web of Science Citations: 5.
TEODORO, GUILHERME RODRIGUES; LACERDA GONTIJO, ALINE VIDAL; BORGES, ALINE CHIODI; TANAKA, MARCIA HIROMI; GOUVEA LIMA, GABRIELA DE MORAIS; SALVADOR, MARCOS JOSE; KOGA-ITO, CRISTIANE YUMI. Gallicacid/hydroxypropyl-beta-cyclodextrin complex: Improving solubility for application on in vitro/in vivo Candida albicans biofilms. PLoS One, v. 12, n. 7 JUL 11 2017. Web of Science Citations: 5.
BRIGHENTI, FERNANDA LOURENCAO; SALVADOR, MARCOS JOSE; LACERDA GONTIJO, ALINE VIDAL; BOTAZZO DELBEM, ALBERTO CARLOS; BOTAZZO DELBEM, ADINA CLEIA; SOARES, CRISTINA PACHECO; CARVALHO DE OLIVEIRA, MARIA ALCIONEIA; GIRONDI, CAMILA MIORELLI; KOGA-ITO, CRISTIANE YUMI. Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms. FUTURE MICROBIOLOGY, v. 12, n. 1, p. 15-27, JAN 2017. Web of Science Citations: 6.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.