Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms

Author(s)

Brighenti, Fernanda Lourencao ; Salvador, Marcos Jose ; Lacerda Gontijo, Aline Vidal ; Botazzo Delbem, Alberto Carlos ; Botazzo Delbem, Adina Cleia ; Soares, Cristina Pacheco ; Carvalho de Oliveira, Maria Alcioneia ; Girondi, Camila Miorelli ; Koga-Ito, Cristiane Yumi

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 12, n. 1, p. 15-27, JAN 2017.
• Web of Science Citations: 6

Abstract

Aim: This study screened plants from Brazilian Pantanal for Candida albicans antibiofilm activity. Material \& Methods: Sixty extracts were obtained from ten plants using different extraction methods. Antifungal activity was assessed. Effects on biofilm inhibition and disruption and cytotoxicity were also evaluated. The most active extract was chemically characterized. Results: Buchenavia tomentosa ethanolic extract showed noticeable antifungal activity and was selected for biofilm experiments. Subinhibitory concentration of extract inhibited fungal adhesion. Maximum killing reached 90% of C. albicans cells in suspension and 65% of cells in biofilms. The active extract was noncytotoxic. Chemical characterization showed the presence of phenols. Ellagic and gallic acids showed activity on C. albicans. Conclusion: B. tomentosa extract and its isolated compound, ellagic acid, presented antibiofilm activity and low toxicity. (AU)

• FAPESP's process: 14/09666-4 - DEPLOYMENT OF ASSAYS FOR EVALUATION OF BIOPHARMACEUTIC CHARACTERISTICS AND BIOACTIVITY OF COMPOUNDS AND BIOACTIVE PRODUCTS USING IN VITRO MODELS OF INVASIVE CANDIDIASIS
• Grantee: Aline Vidal Lacerda Gontijo
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 08/04114-2 - Screening of plants with anticariogenic properties from Brazilian Pantanal
• Grantee: Fernanda Lourenção Brighenti
• Support type: Scholarships in Brazil - Post-Doctorate