Partial lipectomy reduces dimethylhydrazine-induced carcinogenic initiation in the colon of rats

Autor(es)

Kannen, Vinicius ^[1] ; Silveira Moreira, Mauro Cesar ^[2] ; Waaga-Gasser, Ana Maria ^[1] ; Modiano, Patricia ^[2] ; Elias Junior, Jorge ^[3] ; Fernandes, Cleverson R. ^[2] ; Garcia, Sergio B. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Wurzburg, Dept Surg Mol Oncol & Immunol 1, D-97080 Wurzburg - Germany

^[2] Univ Sao Paulo, Dept Pathol, BR-14049 Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Div Radiol, Med Sch Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicology; v. 316, p. 9-13, FEB 28 2014.
• Citações Web of Science: 8

Resumo

This study investigated whether visceral adipose tissue directly modulates the development of preneoplastic lesions in the colon of carcinogen-treated rats. Wistar rats (n = 64) were randomly assigned to 8 experimental groups in two experiments. In one experiment, 32 rats were exposed or not to either carcinogen treatment (dimethylhydrazine, DMH; 125 mg/kg) or high-fat diet (standard chow enriched with 14% lard) or both for 56 days. In a second experiment, 32 rats were exposed to a carcinogen or they underwent partial lipectomy or both for 30 days (partial lipectomy groups underwent ablation of mesenteric and parametrial fat pads, whereas sham groups did not; all rats were fed with standard chow). Colon was collected for histopathological analysis. After 56 experimental days a high-fat diet increased carcinogenic mutations in the colonic epithelia. Partial lipectomy reduced weight gain in carcinogen-exposed rats and decreased the de novo formation of mesenteric and parametrial fat pads. Partial lipectomy significantly inhibited the mutational process after 30 days: there were fewer colonic preneoplastic lesions and less proliferation, apoptosis, and inflammation. These data suggest that visceral adipose tissue promotes colon carcinogenesis and enhances the establishment and expansion of genetically mutated cells in colonic epithelia. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 01/14227-0 - Estudo da incidência, morfologia, alterações do gene APC e instabilidade de microsatélites nos focos de criptas aberrantes induzidos por dimetilhidrazina no megacólon experimental em ratos
• Beneficiário: Sérgio Britto Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular