Peptide diversity in the venom of the social wasp Polybia paulista (Hymenoptera): A comparison of the intra- and inter-colony compositions

Autor(es)

Dias, Nathalia Baptista ^[1] ; de Souza, Bibiana Monson ^[1] ; Gomes, Paulo Cesar ^[1] ; Palma, Mario Sergio ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Ctr Studies Social Insects, Inst Biosci, Rio Claro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Peptides; v. 51, p. 122-130, JAN 2014.
• Citações Web of Science: 12

Resumo

The venoms of the social wasps evolved to be used as defensive tools to protect the colonies of these insects against the attacks of predators. Previous studies estimated the presence of a dozen peptide components in the venoms of each species of these insects, which altogether comprise up to 70% of the weight of freeze-dried venoms. In the present study, an optimized experimental protocol is reported that utilizes liquid chromatography coupled to electrospray ionization mass spectrometry for the detection of peptides in the venom of the social wasp Polybia paulista; peptide profiles for both intra- and inter-colonial comparisons were obtained using this protocol. The results of our study revealed a surprisingly high level of intra- and inter-colonial variability for the same wasp species. We detected 78-108 different peptides in the venom of different colonies of P. paulista in the molar mass range from 400 to 3000 Da; among those, only 36 and 44 common peptides were observed in the inter- and intra-colony comparisons, respectively. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 09/11155-0 - O uso de um sistema LC-ESI-IT-Tof/MS e MSn na prospecção de novos componentes peptídicos do veneno da vespa social Polybia paulista.
• Beneficiário: Nathalia Baptista Dias
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/51684-1 - Biologia de sistemas como estratégia experimental para a descoberta de novos produtos naturais na fauna de artrópodes peçonhentos do Estado de São Paulo
• Beneficiário: Mario Sergio Palma
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático