Mendelian inheritance, genetic linkage, and genotypic disequilibrium at nine microsatellite loci of Cariniana legalis (Mart.) O. Kuntze

Autor(es)

Tambarussi, E. V. ^[1] ; Vencovsky, R. ^[1] ; Freitas, M. L. M. ^[2] ; Sebbenn, A. M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[2] Inst Florestal Sao Paulo, Sao Paulo - Brazil

^[3] Inst Florestal Sao Paulo, Estacao Expt Tupi, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 12, n. 4, p. 5442-5457, 2013.
• Citações Web of Science: 8

Resumo

Cariniana legalis is one of the largest tropical trees with a wide distribution in the Brazilian Atlantic rainforest. We investigated the Mendelian inheritance, genetic linkage, and genotypic disequilibrium at seven microsatellite loci specifically isolated for C. legalis, and at two previously developed heterologous microsatellite loci. Forty to 100 open-pollinated seeds were collected from 22 seed-trees in two populations. Using the Bonferroni correction, no remarkable deviations from the expected Mendelian segregation, linkage, or genotypic disequilibrium were detected in the nine loci studied. Only 3.7% of the tests were significant for investigations of the Mendelian proportions. On the other hand, only 2.8% of tests for linkage detection showed significance. In addition, among all pairwise tests used for investigating linkage disequilibrium, significance was found in 9.7% of the locus pairs. Our results show clear evidence that the nine simple sequence repeat loci can be used without restriction in genetic diversity, mating system, and parentage analyses. (AU)

• Processo FAPESP: 10/10704-7 - Fluxo gênico contemporâneo, sistema de cruzamento e distribuição espacial de genótipos em população fragmentada de jequitibá-rosa (Cariniana legalis Mart. Kuntze) por marcadores microssatélites e análise de parentesco
• Beneficiário: Alexandre Magno Sebbenn
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/12354-3 - Fluxo gênico contemporâneo, sistema de reprodução e estrutura espacial de genótipos em população fragmentada de jequitibá-rosa (Cariniana legalis Mart. O. Kuntze) utilizando marcadores microssatélites.
• Beneficiário: Evandro Vagner Tambarussi
• Modalidade de apoio: Bolsas no Brasil - Doutorado