Cyclic-di-GMP levels affect Pseudomonas aeruginosa fitness in the presence of imipenem

Autor(es)

Nicastro, Gianlucca G. ^[1] ; Kaihami, Gilberto H. ^[1] ; Pereira, Thays O. ^[1] ; Meireles, Diogo A. ^[1] ; Groleau, Marie-Christine ^[2] ; Deziel, Eric ^[2] ; Baldini, Regina L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil

^[2] INRS, Inst Armand Frappier, Laval, PQ - Canada

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL MICROBIOLOGY; v. 16, n. 5, p. 1321-1333, MAY 2014.
• Citações Web of Science: 7

Resumo

A large number of genes coding for enzymes predicted to synthesize and degrade 3 `-5 `-cyclic diguanylic acid (c-di-GMP) is found in most bacterial genomes and this dinucleotide emerged as an intracellular signal-controlling bacterial behaviour. An association between high levels of c-di-GMP and antibiotic resistance may be expected because c-di-GMP regulates biofilm formation and this mode of growth leads to enhanced antibiotic resistance. However, a clear understanding of this correlation has not been established. We found that increased levels of c-di-GMP in Pseudomonas aeruginosa improve fitness in the presence of imipenem, even when grown as planktonic cells. P. aeruginosa post-transcriptionally regulates the amounts of five porins in response to c-di-GMP, including OprD, responsible for imipenem uptake. Cells with low c-di-GMP levels are consequently more sensitive to this antibiotic. Main efflux pumps or beta-lactamase genes did not show altered mRNA levels in P. aeruginosa strains with modified different c-di-GMP concentrations. Together, our findings show that c-di-GMP levels modulate fitness of planktonic cultures in the presence of imipenem. (AU)

• Processo FAPESP: 09/08198-9 - Busca por alvos de regulação pelo segundo mensageiro c-diGMP em Pseudomonas aeruginosa
• Beneficiário: Gianlucca Gonçalves Nicastro
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/03121-2 - Estudo da função do gene PA14_41070 de Pseudomonas aeruginosa, envolvido em patogenicidade e "quorum sensing"
• Beneficiário: Diogo de Abreu Meireles
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/08841-9 - Estudo comparativo de mecanismos de regulação pelo segundo mensageiro c-di-GMP em Pseudomonas e Xanthomonas
• Beneficiário: Regina Lúcia Baldini
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/09211-9 - Caracterização da função e regulação de genes relacionados à virulência da proteobactéria Pseudomonas aeruginosa
• Beneficiário: Regina Lúcia Baldini
• Linha de fomento: Auxílio à Pesquisa - Regular