Low level laser therapy reduces acute lung inflammation in a model of pulmonary and extrapulmonary LPS-induced ARDS

Autor(es):Mostrar menos -

Oliveira, Jr., Manoel Carneiro ^[1] ; Greiffo, Flavia Regina ^[1] ; Rigonato-Oliveira, Nicole Cristine ^[1] ; Alberca Custodio, Ricardo Wesley ^[1] ; Silva, Vanessa Roza ^[1] ; Damaceno-Rodrigues, Nilsa Regina ^[2] ; Almeida, Francine Maria ^[3] ; Albertini, Regiane ^[1] ; Lopes-Martins, Rodrigo Alvaro B. ^[1] ; Franco de Oliveira, Luis Vicente ^[1] ; Camillo de Carvalho, Paulo de Tarso ^[1] ; Ligeiro de Oliveira, Ana Paula ^[1] ; Leal, Jr., Ernesto Cesar P. ^[1] ; Vieira, Rodolfo P. ^[1]

Afiliação do(s) autor(es)

^[1] Nove Julho Univ, BR-01504000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Pathol LIM 59, BR-01246000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, BR-01246000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 134, p. 57-63, MAY 5 2014.
• Citações Web of Science: 21

Resumo

The present study aimed to investigate the effects low level laser therapy (LLLT) in a LPS-induced pulmonary and extrapulmonary acute respiratory distress syndrome (ARDS) in BALB/c mice. Laser (830 nm laser, 9 J/cm(2), 35 mW, 80 s per point, 3 points per application) was applied in direct contact with skin, 1 h after LPS administration. Mice were distributed in control (n = 6; PBS), ARDS IT (n = 7; LPS orotracheally 10 mu g/mouse), ARDS IP (n = 7; LPS intra-peritoneally 100 mu g/mouse), ARDS IT + Laser (n = 9; LPS intra-tracheally 10 mu g/mouse), ARDS IP + Laser (n = 9; LPS intra-peritoneally 100 mu g/mouse). Twenty-four hours after last LPS administration, mice were studied for pulmonary inflammation by total and differential cell count in bronchoalveolar lavage (BAL), cytokines (IL-1 beta, IL-6, KC and TNF-alpha) levels in BAL fluid and also by quantitative analysis of neutrophils number in the lung parenchyma. LLLT significantly reduced pulmonary and extrapulmonary inflammation in LPS-induced ARDS, as demonstrated by reduced number of total cells (p < 0.001) and neutrophils (p < 0.001) in BAL, reduced levels of IL-1 beta, IL-6, KC and TNF-alpha in BAL fluid and in serum (p < 0.001), as well as the number of neutrophils in lung parenchyma (p < 0.001). LLLT is effective to reduce pulmonary inflammation in both pulmonary and extrapulmonary model of LPS-induced ARDS. (C) 2014 Elsevier B.V. All rights reserved. (AU)

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