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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Low level laser therapy reduces acute lung inflammation in a model of pulmonary and extrapulmonary LPS-induced ARDS

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Oliveira, Jr., Manoel Carneiro [1] ; Greiffo, Flavia Regina [1] ; Rigonato-Oliveira, Nicole Cristine [1] ; Alberca Custodio, Ricardo Wesley [1] ; Silva, Vanessa Roza [1] ; Damaceno-Rodrigues, Nilsa Regina [2] ; Almeida, Francine Maria [3] ; Albertini, Regiane [1] ; Lopes-Martins, Rodrigo Alvaro B. [1] ; Franco de Oliveira, Luis Vicente [1] ; Camillo de Carvalho, Paulo de Tarso [1] ; Ligeiro de Oliveira, Ana Paula [1] ; Leal, Jr., Ernesto Cesar P. [1] ; Vieira, Rodolfo P. [1]
Total Authors: 14
[1] Nove Julho Univ, BR-01504000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Pathol LIM 59, BR-01246000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, BR-01246000 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 21

The present study aimed to investigate the effects low level laser therapy (LLLT) in a LPS-induced pulmonary and extrapulmonary acute respiratory distress syndrome (ARDS) in BALB/c mice. Laser (830 nm laser, 9 J/cm(2), 35 mW, 80 s per point, 3 points per application) was applied in direct contact with skin, 1 h after LPS administration. Mice were distributed in control (n = 6; PBS), ARDS IT (n = 7; LPS orotracheally 10 mu g/mouse), ARDS IP (n = 7; LPS intra-peritoneally 100 mu g/mouse), ARDS IT + Laser (n = 9; LPS intra-tracheally 10 mu g/mouse), ARDS IP + Laser (n = 9; LPS intra-peritoneally 100 mu g/mouse). Twenty-four hours after last LPS administration, mice were studied for pulmonary inflammation by total and differential cell count in bronchoalveolar lavage (BAL), cytokines (IL-1 beta, IL-6, KC and TNF-alpha) levels in BAL fluid and also by quantitative analysis of neutrophils number in the lung parenchyma. LLLT significantly reduced pulmonary and extrapulmonary inflammation in LPS-induced ARDS, as demonstrated by reduced number of total cells (p < 0.001) and neutrophils (p < 0.001) in BAL, reduced levels of IL-1 beta, IL-6, KC and TNF-alpha in BAL fluid and in serum (p < 0.001), as well as the number of neutrophils in lung parenchyma (p < 0.001). LLLT is effective to reduce pulmonary inflammation in both pulmonary and extrapulmonary model of LPS-induced ARDS. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/23305-9 - Effects of aerobic physical training on the chronic allergic pulmonary inflammation in mice: role of purinergic signaling
Grantee:Flavia Regina Greiffo
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
Grantee:Rodolfo de Paula Vieira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/21519-1 - Is the leukotrienes via contributes to the anti-inflammatory effects of aerobic exercise in asthma?
Grantee:Ricardo Wesley Alberca Custódio
Support type: Scholarships in Brazil - Master