Bioprospection of Petit Verdot grape pomace as a source of anti-inflammatory compounds

Autor(es)

Denny, C. ^[1] ; Lazarini, J. G. ^[1] ; Franchin, M. ^[1] ; Melo, P. S. ^[2] ; Pereira, G. E. ^[3] ; Massarioli, A. P. ^[2] ; Moreno, I. A. M. ^[2] ; Paschoal, J. A. R. ^[4] ; Alencar, S. M. ^[2] ; Rosalen, P. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, Campinas, SP - Brazil

^[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agri Food Ind Food & Nutr, BR-05508 Sao Paulo - Brazil

^[3] Embrapa Grape & Wine Trop Semi Arid, Brazilian Agr Res Corp, Petrolina, PE - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Functional Foods; v. 8, p. 292-300, MAY 2014.
• Citações Web of Science: 14

Resumo

The aim of this study was to evaluate the anti-inflammatory activity of Petit Verdot Extract and hexane, chloroform and ethyl acetate fractions obtained from grape pomace, in addition to identifying active compounds. The PVE and EAF reduced significantly paw edema and neutrophil migration when compared with control groups. The PVE reduced levels of TNF-alpha and IL1-beta in the peritoneal fluid, whereas the EAF did not reduce cytokines significantly. Two hydroxybenzoic acids, two proanthocyanidins, three flavan-3-ol monomers and three anthocyanins were identified in the PVE and EAF by LC-MS/MS. The stilbene transresveratrol was identified only in the EAF. The phenolic compounds were quantified using HPLC-DAD analysis, except for the stilbenes, which were not sensible for the detection by the method. Since PVE and EAF showed significantly anti-inflammatory effects and high concentration of phenolic compounds, we concluded that Petit Verdot pomace could be an interesting source of anti-inflammatory bioactives. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/12640-9 - Identificação de antioxidantes naturais em resíduos agroindustriais: isolamento e aplicação em matriz alimentar de base lipídica
• Beneficiário: Priscilla Siqueira Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado