Chloroquine interferes with dengue-2 virus replication in U937 cells

Autor(es)

Silva Farias, Kleber Juvenal ^{[1, 2, 3]} ; Lima Machado, Paula Renata ^{[1, 2, 3]} ; de Almeida Junior, Renato Ferreira ^[4] ; de Aquino, Ana Alice ^[4] ; Lopes da Fonseca, Benedito Antonio ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Internal Med, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Program Grad Studies Appl Microbiol & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Virol Res Ctr, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Univ Fed Rio Grande do Norte, Biosci Ctr, Program Grad Studies Biol Sci, BR-59072970 Natal, RN - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIOLOGY AND IMMUNOLOGY; v. 58, n. 6, p. 318-326, JUN 2014.
• Citações Web of Science: 16

Resumo

The objective of this study was to investigate the use of chloroquine (CLQ) as an antiviral agent against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of U937 cells infected with dengue virus type 2 (DENV-2). Viral replication was assessed by quantification of virus produced through detection of copy numbers of DENV-2 RNA, plaque assay and indirect immunofluorescence. qRT-PCR and plaque assays were used to quantify the DENV-2 load in infected U937 cells after CLQ treatment. It was found that a dose of 50mg/mL of CLQ was not toxic to the cells and resulted in significantly less virus production in infected U937 cells than occurred in untreated cells. In the present work, CLQ was effective against DENV-2 replication in U937 cells, and also caused a statistically significant reduction in expression of proinflammatory cytokines. The present study indicates that CLQ may be used to reduce viral yield in U937 cells. (AU)

• Processo FAPESP: 05/04450-4 - Analise da expressao quantitativa dos genes codificadores das proteinas do virus dengue-2 durante a reaplicacao viral em condicoes normais e sob a acao de drogas.
• Beneficiário: Benedito Antônio Lopes da Fonseca
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/55789-4 - Analise da acao da cloroquina sob a replicacao do virus dengue-2 em celulas de linhagem monociticas e em macacos aotus.
• Beneficiário: Kleber Juvenal Silva Farias
• Modalidade de apoio: Bolsas no Brasil - Doutorado