Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria

Autor(es)

Elizabeth M A Rabelo-Gonçalves ^[1] ; Nancy F Nishimura ^[2] ; José Murilo R Zeitune ^[3]

Afiliação do(s) autor(es)

^[1] State University of Campinas-UNICAMP. Gastrocenter. Laboratory of Bacteriology - Brasil

^[2] State University of Campinas-UNICAMP. Gastrocenter. Laboratory of Bacteriology - Brasil

^[3] State University of Campinas-UNICAMP. Gastrocenter. Laboratory of Bacteriology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Biological Research; v. 38, n. 1, p. 101-109, 2005-00-00.

Resumo

An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori induced lymphomonocytic infiltration that was present from days 7 to 60 in group GA. In group GB, it was only present from days 14 to 35. In conclusion, our data suggested that freezing altered pathogenic properties of H. pylori and probably inhibited expression of bacterial antigens and consequently the establishment and maintenance of infection. Although the animals developed mild duodenitis and gastritis, the BALB/c mouse is not susceptible to developing peptic ulcers during H. pylori infection (AU)

• Processo FAPESP: 99/07821-0 - Estabelecimento de um modelo experimental murino para infecção com Helicobacter pylori no Brasil: aspectos histopatológicos
• Beneficiário: Elizabeth Maria Afonso Rabelo Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Mestrado