Anti-inflammatory effect of parenteral fish oil lipid emulsion on human activated mononuclear leukocytes

Autor(es)

Jacintho, T. Manzoni ^[1] ; Gotho, H. ^[2] ; Gidlund, A. ^[2] ; Marques, C. Garcia ^[1] ; Torrinhas, R. ^[1] ; Sales, Ma Mirtes ^[3] ; Waitzberg, D. Linetzky ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, METANUTRI Lab Fisiol & Disturbios Esfincterianos, BR-05508 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Lab Soroepidemiol & Imunobiol LIM38, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Div Citometria HCFMUSP, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRICION HOSPITALARIA; v. 24, n. 3, p. 288-296, MAY-JUN 2009.
• Citações Web of Science: 8

Resumo

Background & aim: To compare the effect of fish oilbased (FO) lipid emulsions (LE) for parenteral administration with standard LE and a new FO containing LE composed of four different oils on the antigen presentation and inflammatory variables. Methods: Phytohemagglutinin (PHA) activated human mononuclear leukocytes were cultured with different LE - Control: without LE; SO: soybean oil; SO/FO: soybean and FO (4:1); MCT/SO: medium chain triglycerides and SO (1:1); MCT/SO/FO: MCT/SO and FO (4:1) and SMOF: a new LE containing FO. Cytokine production was evaluated by ELISA, the expression of antigen-presenting and co-stimulatory surface molecules were analyzed by flow cytometry and lymphocyte proliferation was assessed by H³-Thymidine incorporation, after tetanus toxoid-induced activation. Results: All LE decreased the HLA-DR and increased CD28 and CD152 expression on monocytes/macrophages and lymphocytes surface (p < 0.05). SO/FO and MCT/SO/FO decreased lymphocyte proliferation (p<0.05). All LE decreased IL-2 production, but this effect was enhanced with MCT/SO/FO and SMOF (p < 0.05). MCT/SO/FO decreased IL-6 and increased IL-10, whereas SO had the opposite effect (p < 0.05). Conclusion: FO LE inhibited lymphocyte proliferation and had an anti-inflammatory effect. These effects seem to be enhanced when FO is mixed with MCT/SO. SMOF had a neutral impact on lymphocyte proliferation and IL-6 and IL-10 production. (AU)

• Processo FAPESP: 04/04225-8 - Efeitos de diferentes emulsões lipídicas sobre a expressão de moléculas envolvidas no processo de apresentação de antígenos em células mononucleares humanas
• Beneficiário: Dan Linetzky Waitzberg
• Modalidade de apoio: Auxílio à Pesquisa - Regular