Antiproliferative effects of Tubi-bee propolis in glioblastoma cell lines

Autor(es)

Borges, Kleiton Silva ^[1] ; Brassesco, Maria Sol ^[2] ; Scrideli, Carlos Alberto ^[2] ; Egea Soares, Ademilson Espencer ^[1] ; Tone, Luiz Gonzaga ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14048900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Pediat, Escola Med Ribeirao Preto, Div Pediat Oncol, BR-14048900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 34, n. 2, p. 310-314, 2011.
• Citações Web of Science: 17

Resumo

Propolis is a resin formed by a complex chemical composition of substances that bees collect from plants. Since ancient times, propolis has been used in folk medicine, due to its biological properties, that include antimicrobial, anti-inflammatory, antitumoral and immunomodulatory activities. Glioblastoma is the most common human brain tumor. Despite the improvements in GBM standard treatment, patients' prognosis is still very poor. The aim of this work was to evaluate in vitro the Tubi-bee propolis effects on human glioblastoma (U251 and U343) and fibroblast (MRC-5) cell lines. Proliferation, clonogenic capacity and apoptosis were analyzed after treatment with 1 mg/mL and 2 mg/mL propolis concentrations for different time periods. Additionally, glioblastoma cell lines were submitted to treatment with propolis combined with temozolomide (TMZ). Data showed an antiproliferative effect of tubi-bee propolis against glioblastoma and fibroblast cell lines. Combination of propolis with TMZ had a synergic antiproliferative effect. Moreover, propolis caused decrease in colony formation in glioblastoma cell lines. Propolis treatment had no effects on apoptosis, demonstrating a cytostatic action. Further investigations are needed to elucidate the molecular mechanism of the antitumor effect of propolis, and the study of its individual components may reveal specific molecules with antiproliferative capacity. (AU)

• Processo FAPESP: 09/50118-2 - Investigacao de novos alvos terapeuticos envolvidos com a instabilidade genomica em glioblastoma.
• Beneficiário: Luiz Gonzaga Tone
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/04827-3 - Análise de aneuploidias por hibridação genômica comparativa (CGH) em pacientes pediátricos portadores de leucemia linfóide aguda
• Beneficiário: María Sol Brassesco Annichini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/08699-5 - Mecanismos envolvidos com a inibição de aurora-quinases em carcinoma de adrenal
• Beneficiário: Kleiton Silva Borges
• Modalidade de apoio: Bolsas no Brasil - Doutorado