The in vitro maintenance of clock genes expression within the rat pineal gland under standard and norepinephrine-synchronized stimulation

Autor(es)

Andrade-Silva, Jessica ^[1] ; Cipolla-Neto, Jose ^[1] ; Peliciari-Garcia, Rodrigo A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NEUROSCIENCE RESEARCH; v. 81-82, p. 1-10, APR-MAY 2014.
• Citações Web of Science: 5

Resumo

Although the norepinephrine (NE) synchronization protocol was proved to be an important procedure for further modulating in vitro pineal melatonin synthesis, the maintenance of clock genes under the same conditions remained to be investigated. The aim of this study was to investigate the maintenance of the clock genes expression in pineal gland cultures under standard and NE-synchronized stimulation. The glands were separated into three experimental groups: Control, Standard (acute NE-stimulation), and NE-synchronized. The expression of Bmal1, Per2, Cry2, Rev-erb alpha, the clock controlled gene Dbp and Arylalkylamine-N-acetyltransferase were investigated, as well as melatonin content. No oscillations were observed in the expression of the investigated genes from the control group. Under Standard NE stimulation, the clock genes did not exhibit a rhythmic pattern of expression. However, in the NE-synchronized condition, a rhythmic expression pattern was observed in all cases. An enhancement in pineal gland responsiveness to NE stimulation, reflected in an advanced synthesis of melatonin was also observed. Our results reinforce our previous hypothesis that NE synchronization of pineal gland culture mimics the natural rhythmic release of NE in the gland, increasing melatonin synthesis and keeping the pineal circadian clock synchronized, ensuring the fine adjustments that are relied in the clockwork machinery. (C) 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. (AU)

• Processo FAPESP: 07/07790-6 - Papel da insulina na regulação da síntese de melatonina pela glândula pineal: características funcionais e mecanismos de ação
• Beneficiário: Rodrigo Antonio Peliciari Garcia
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/52920-0 - O papel da melatonina no controle do metabolismo energético: ações centrais, periféricas e a regulação circadiana da função metabólica pineal, diabetes, obesidade e envelhecimento
• Beneficiário: José Cipolla Neto
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/04359-8 - Papel da temporização noradrenérgica na regulação da síntese de melatonina pela glândula pineal em cultura: características funcionais e mecanismos de ação
• Beneficiário: Jéssica Andrade da Silva
• Linha de fomento: Bolsas no Brasil - Mestrado