Effect of HeNe Laser Irradiation on Extracellular Matrix Deposition and Expression of Cytokines and Chemokines in Paracoccidioidomycotic Lesions

Autor(es)

Nagib, Patricia R. A. ^[1] ; Gameiro, Jacy ^[2] ; da Costa, Thiago Alves ^[3] ; Di Gangi, Rosaria ^[4] ; Ribeiro, Julia da Silva ^[5] ; Paulino, Luciana Campos ^[6] ; Verinaud, Liana ^[7]

Afiliação do(s) autor(es)

^[1] Univ Fed Goias. Inst Trop Pathol & Publ Hlth

^[2] UFJF. Dept Microbiol Immunol & Parasitol

^[3] State Univ Campinas UNICAMP. Inst Biol

^[4] State Univ Campinas UNICAMP. Inst Biol

^[5] State Univ Campinas UNICAMP. Inst Biol

^[6] UFABC. Sao Paulo

^[7] State Univ Campinas UNICAMP. Inst Biol

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 86, n. 4, p. 920-924, JUL-AUG 2010.
• Citações Web of Science: 1

Resumo

Paracoccidioidomycosis is the most prevalent human mycosis in Latin America. Cutaneous lesions are extremely painful and sensitive, and current treatment with antifungal drugs is lengthy and may cause side effects to patients. In this perspective, the helium-neon (HeNe) laser emerges as a novel therapy form due to its ability to heal wounds without changing cell function. In this work, we evaluate the effects of HeNe laser irradiation on extracellular matrix deposition and expression of cytokines and chemokines in cutaneous lesions caused by experimental infection of Balb/c mice. Our results showed decreased levels of pro-inflammatory interleukin (IL)-17 and tumor necrosis factor-alpha, and of anti-inflammatory IL-10 cytokines in lesions exposed to HeNe laser irradiation. Chemokines CCL3 and CXCL10 showed decreased levels in laser-treated lesions, but no significant difference was observed in relation to CCL5 expression. We also detected decreased density of fibronectin and laminin in HeNe laser-treated lesions. Data presented herein support the validity of our previous results suggesting positive effects of HeNe laser in accelerating wound healing in this experimental model. We believe that HeNe laser is a new nonharmful strategy that may be used as adjuvant and/or alternative therapy for improving treatment of paracoccidioidomycotic lesions. (AU)

• Processo FAPESP: 05/55234-0 - Análise dos efeitos do laser HeNe sobre a matriz extracelular e angiogênese em lesões paracoccidiodomicóticas
• Beneficiário: Liana Maria Cardoso Verinaud
• Modalidade de apoio: Auxílio à Pesquisa - Regular