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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation

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Autor(es):
Rodrigues de Siqueira, Ana Claudia [1] ; da Rosa, Nathalia Gonsales [1] ; Souza Motta, Cristina Maria [2] ; Cabral, Hamilton [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14049 Ribeirao Preto, SP - Brazil
[2] Univ Fed Pernambuco, Dept Micol, Ctr Ciencias Biol, Recife, PE - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Brazilian Archives of Biology and Technology; v. 57, n. 4, p. 514-522, JUL-AUG 2014.
Citações Web of Science: 2
Resumo

The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30 degrees C, inoculum 2.0x10(5) spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation. Biochemical characterization of the crude enzymatic extract showed the optimum pH and temperature of 6.5 and 55 degrees C, respectively. The stability at different temperatures and pH values showed that the extract could endure different pH. The evaluation of the ions influence and inhibitors proved that the enzyme required an ion for better activity, which was corroborated with the inhibition of EDTA and PMSF, characterizing serine and/or metallo peptidase. The extract was also tested for specific activities and showed promising results for keratinolytic and collagenolytic activities (0.252 and 0.165 OD/mL, respectively). (AU)

Processo FAPESP: 11/06986-0 - Determinação da especificidade de peptidases isoladas de fungos usando peptídeos FRET como substratos
Beneficiário:Hamilton Cabral
Linha de fomento: Auxílio à Pesquisa - Regular