Conventional PCR for molecular diagnosis of human strongyloidiasis

Autor(es)

Sitta, R. B. ^[1] ; Malta, F. M. ^{[1, 2]} ; Pinho, J. R. ^{[1, 2]} ; Chieffi, P. P. ^{[3, 1]} ; Gryschek, R. C. B. ^[4] ; Paula, F. M. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Gastroenterol LIM 07, BR-05403000 Sao Paulo - Brazil

^[3] Fac Ciencias Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, Hosp Clin, Dept Molestias Infecciosas & Parasitarias LIM 06, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology; v. 141, n. 5, p. 716-721, APR 2014.
• Citações Web of Science: 15

Resumo

Strongyloidiasis is frequently asymptomatic and diagnosis of latent infection is difficult due to limitations of current parasitological and serological methods. This study aimed to verify the use of conventional polymerase chain reaction (PCR) assay for molecular diagnosis of Strongyloides stercoralis infection. Fresh stool samples were obtained from 103 individuals: 33 S. stercoralis positive, 30 positive for other parasites and 40 negative for parasitological methods. These samples were examined by the Lutz, Rugai and agar plate culture methods and conventional PCR assay. Two sets of primers (S. stercoralis species-specific and genus-specific sets), located in the 18S ribosomal RNA gene, were used for PCR. Of the 33 samples positive for S. stercoralis by parasitological methods, 28 (84.8%) were also detected by PCR assay using species-specific primers and 26 (78.8%) using genus-specific primers. Among the stool samples negative by parasitological methods, seven (17.5%) were positive by PCR using species-specific primers and two (5.0%) using genus-specific primers. In conclusion, the conventional PCR assay described in this study using a species-specific primer pair provided a molecular method for S. stercoralis diagnosis in human stool samples. (AU)

• Processo FAPESP: 10/51110-2 - Investigação de técnicas diagnósticas para identificação da infecção por S. stercoralis em pacientes com diferentes formas de imunodepressão
• Beneficiário: Pedro Paulo Chieffi
• Modalidade de apoio: Auxílio à Pesquisa - Regular