Worldwide sequence conservation of transmission-blocking vaccine candidate Pvs230 in Plasmodium vivax

Autor(es):Mostrar menos -

Doi, Masanori ^[1] ; Tanabe, Kazuyuki ^[2] ; Tachibana, Shin-Lchiro ^[2] ; Hamai, Meiko ^[3] ; Tachibana, Mayumi ^[3] ; Mita, Toshihiro ^[4] ; Yagi, Masanori ^[5] ; Zeyrek, Fadile Yildiz ^[6] ; Ferreira, Marcelo U. ^[7] ; Ohmae, Hiroshi ^[8] ; Kaneko, Akira ^{[9, 10]} ; Randrianarivelojosia, Milijaona ^[11] ; Sattabongkot, Jetsumon ^[12] ; Cao, Ya-Ming ^[13] ; Horii, Toshihiro ^[5] ; Torii, Motomi ^{[3, 14]} ; Tsuboi, Takafumi ^{[1, 14, 15]}

Afiliação do(s) autor(es):Mostrar menos -

^[1] Ehime Univ, Cell Free Sci & Technol Res Ctr, Matsuyama, Ehime 7908577 - Japan

^[2] Osaka Univ, Microbial Dis Res Inst, Lab Malariol, Suita, Osaka 5650871 - Japan

^[3] Ehime Univ, Dept Mol Parasitol, Grad Sch Med, Toon, Ehime 7910295 - Japan

^[4] Tokyo Womens Med Univ, Dept Int Affairs & Trop Med, Tokyo 1628666 - Japan

^[5] Osaka Univ, Microbial Dis Res Inst, Dept Mol Protozool, Suita, Osaka 5650871 - Japan

^[6] Harran Univ, Fac Med, Dept Microbiol, Sanliurfa - Turkey

^[7] Univ Sao Paulo, Dept Parasitol, Inst Biomed Sci, Sao Paulo - Brazil

^[8] Natl Inst Infect Dis, Dept Parasitol, Tokyo - Japan

^[9] Karolinska Inst, Isl Malaria Grp, Dept Microbiol Timor & Cell Biol, S-17177 Stockholm - Sweden

^[10] Nagasaki Univ, Global COE, Inst Trop Med, Nagasaki 852 - Japan

^[11] Inst Pasteur Madagascar, Unite Rech Paludisme, Antananarivo - Madagascar

^[12] Armed Forces Res Inst Med Sci, Dept Entomol, Bangkok 10400 - Thailand

^[13] China Med Univ, Dept Immunol, Coll Basic Med Sci, Shenyang 110001 - Peoples R China

^[14] Ehime Univ, Ehime Proteomed Res Ctr, Toon, Ehime 7910295 - Japan

^[15] Ehime Univ, Venture Business Lab, Matsuyama, Ehime 7908577 - Japan

• Tipo de documento: Artigo Científico
• Fonte: Vaccine; v. 29, n. 26, p. 4308-4315, JUN 10 2011.
• Citações Web of Science: 21

Resumo

Pfs230, surface protein of gametocyte/gamete of the human malaria parasite, Plasmodium falciparum, is a prime candidate of malaria transmission-blocking vaccine. Plasmodium vivax has an ortholog of Pfs230 (Pvs230), however, there has been no study in any aspects on Pvs230 to date. To investigate whether Pvs230 can be a vivax malaria transmission-blocking vaccine, we performed evolutionary and population genetic analysis of the Pvs230 gene (pvs230: PVX\_003905). Our analysis of Pvs230 and its orthologs in eight Plasmodium species revealed two distinctive parts: an interspecies variable part (IVP) containing species-specific oligopeptide repeats at the N-terminus and a 7.5 kb interspecies conserved part (ICP) containing 14 cysteine-rich domains. Pvs230 was closely related to its orthologs, Pks230 and Pcys230, in monkey malaria parasites. Analysis of 113 pvs230 sequences obtained from worldwide, showed that nucleotide diversity is remarkably low in the non-repeat 8-kb region of pvs230 (theta pi = 0.00118) with 77 polymorphic nucleotide sites, 40 of which results in amino acid replacements. A signature of purifying selection but not of balancing selection was seen on pvs230. Functional and/or structural constraints may limit the level of polymorphism in pvs230. The observed limited polymorphism in pvs230 should ground for utilization of Pvs230 as an effective transmission-blocking vaccine. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 05/51988-0 - Epidemiologia da malaria: fatores de risco, distribuicao espaco-temporal e resposta ao tratamento em uma coorte rural amazonica.
• Beneficiário: Marcelo Urbano Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/51199-0 - Diversidade genetica, estrutura populacional e dinamica de transmissao de plasmodium vivax na amazonia rural brasileira.
• Beneficiário: Marcelo Urbano Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular