Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL-18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

Autor(es)

Bussmann, Andre Roberto ^[1] ; Marton Filho, Marcos Antonio ^[2] ; Modolo, Marilia Pinheiro ^[2] ; Modolo, Renata Pinheiro ^[3] ; Amado, Patricia ^[2] ; Custodio Domingues, Maria Aparecida ^[4] ; Machado Castiglia, Yara Marcondes ^[5] ; Pinheiro Modolo, Norma Sueli ^[5]

Afiliação do(s) autor(es)

^[1] UNESP, Botucatu Med Sch, Postgrad Program Anaesthesiol, BR-18618970 Botucatu, SP - Brazil

^[2] UNESP, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil

^[3] ABC, Sch Med, Santo Andre, SP - Brazil

^[4] UNESP, Botucatu Med Sch, Dept Pathol, BR-18618970 Botucatu, SP - Brazil

^[5] UNESP, Botucatu Med Sch, Dept Anaesthesiol, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 29, n. 8, p. 515-521, AUG 2014.
• Citações Web of Science: 7

Resumo

PURPOSE: To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM-1 and IL-18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury. METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg.kg(-1).d(-1); and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg.kg(-1).d(-1). The NGALp, NGALu, KIM-1, IL-18 and creatinine levels and the kidney histology were analysed. The significance level was established as p<0.05. RESULTS: Creatinine level increased in all the groups, with A approximate to C > S approximate to CA. The NGALp, NGALu and IL-18 levels exhibited similar behaviour in all the groups. KIM-1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA. CONCLUSION: Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury. (AU)

• Processo FAPESP: 12/13604-9 - Efeitos do alopurinol na proteção renal por meio da dosagem plasmática de NGAL: estudo em modelo experimental de lesão de isquemia e reperfusão em ratos sob anestesia inalatória
• Beneficiário: Norma Sueli Pinheiro Módolo
• Modalidade de apoio: Auxílio à Pesquisa - Regular