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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL-18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

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Author(s):
Bussmann, Andre Roberto [1] ; Marton Filho, Marcos Antonio [2] ; Modolo, Marilia Pinheiro [2] ; Modolo, Renata Pinheiro [3] ; Amado, Patricia [2] ; Custodio Domingues, Maria Aparecida [4] ; Machado Castiglia, Yara Marcondes [5] ; Pinheiro Modolo, Norma Sueli [5]
Total Authors: 8
Affiliation:
[1] UNESP, Botucatu Med Sch, Postgrad Program Anaesthesiol, BR-18618970 Botucatu, SP - Brazil
[2] UNESP, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil
[3] ABC, Sch Med, Santo Andre, SP - Brazil
[4] UNESP, Botucatu Med Sch, Dept Pathol, BR-18618970 Botucatu, SP - Brazil
[5] UNESP, Botucatu Med Sch, Dept Anaesthesiol, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Acta Cirurgica Brasileira; v. 29, n. 8, p. 515-521, AUG 2014.
Web of Science Citations: 7
Abstract

PURPOSE: To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM-1 and IL-18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury. METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg.kg(-1).d(-1); and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg.kg(-1).d(-1). The NGALp, NGALu, KIM-1, IL-18 and creatinine levels and the kidney histology were analysed. The significance level was established as p<0.05. RESULTS: Creatinine level increased in all the groups, with A approximate to C > S approximate to CA. The NGALp, NGALu and IL-18 levels exhibited similar behaviour in all the groups. KIM-1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA. CONCLUSION: Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury. (AU)

FAPESP's process: 12/13604-9 - Effects of allopurinol in renal protection by measurement of plasma NGAL: a study in an experimental model of ischemia and reperfusion in rats under ether anesthesia
Grantee:Norma Sueli Pinheiro Módolo
Support Opportunities: Regular Research Grants