CA88, a nuclear repetitive DNA sequence identified in Schistosoma mansoni, aids in the genotyping of nine Schistosoma species of medical and veterinary importance

Autor(es)

Diana Bahia ; Nilton B Rodrigues ; Flávio Marcos G Araújo ^[3] ; Álvaro José Romanha ^[4] ; Jerônimo C Ruiz ^[5] ; David A Johnston ^[6] ; Guilherme Oliveira

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 105, n. 4, p. 391-397, 2010-07-00.

Resumo

CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma mansoni. The assembled S. mansoni sequence, which contains the CA88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. In addition, CA88 also possesses an internal CA microsatellite, identified as SmBr18. Both PCR and BLAST analysis have been used to analyse and confirm the CA88 sequence in other S. mansoni sequences in the public database. PCR-acquired nuclear repetitive DNA sequence profiles from nine Schistosoma species were used to classify this organism into four genotypes. Included among the nine species analysed were five sequences of both African and Asian lineages that are known to infect humans. Within these genotypes, three of them refer to recognised species groups. A panel of four microsatellite loci, including SmBr18 and three previously published loci, has been used to characterise the nine Schistosoma species. Each species has been identified and classified based on its CA88 DNA fingerprint profile. Furthermore, microsatellite sequences and intra-specific variation have also been observed within the nine Schistosoma species sequences. Taken together, these results support the use of these markers in studying the population dynamics of Schistosoma isolates from endemic areas and also provide new methods for investigating the relationships between different populations of parasites. In addition, these data also indicate that Schistosoma magrebowiei is not a sister taxon to Schistosoma mattheei, prompting a new designation to a basal clade. (AU)

• Processo FAPESP: 07/50551-2 - Identificação e caracterização molecular de proteínas quinases de Trypanosoma cruzi para o estudo da comunicação celular, modelagem molecular e desenho de drogas inibidoras: estudo dos parceiros das vias de sinalização focado na invasão de EA
• Beneficiário: Diana Bahia
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/54831-0 - Identificação e caracterização molecular de proteínas quinases de Trypanosoma cruzi para o estudo da comunicação celular, modelagem molecular e desenho de drogas inibidoras: estudo dos parceiros das vias de sinalização focado na invasão de mastigotas extracelulares
• Beneficiário: Diana Bahia
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores