The fate of chrysotile-induced multipolar mitosis and aneuploid population in cultured lung cancer cells

• Processo: 11/05967-1
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Data de Início da vigência: 01 de setembro de 2011
• Data de Término da vigência: 29 de fevereiro de 2012
• Área do conhecimento: Interdisciplinar
• Pesquisador responsável: Glaucia Maria Machado Santelli
• Beneficiário: Glaucia Maria Machado Santelli
• Instituição Sede: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Assunto(s): Aneuploidia Imagem com lapso de tempo Mitose Crisotila Neoplasias pulmonares Publicações de divulgação científica Artigo científico
• Palavra(s)-Chave do Pesquisador: aneuploidy | chysotile | multipolar mitisis | time lapse microscopy | biologia celular

Resumo

Chrysotile is one of the six types of asbestos, and it is the only one that can still be commercialized in many countries. Exposure to other types of asbestos has been associated with serious diseases, such as lung carcinomas and pleural mesotheliomas. The association of chrysotile exposure with disease is controversial. However, in vitro studies show the mutagenic potential of chrysotile, which can induces DNA and cell damage. The present work aimed to analyze alterations in lung small cell carcinoma cultures after 48 h of chrysotile exposure, followed by 2, 4 and 8 days of recovery in fiber-free culture medium. Some alterations, such as aneuploid cell formation, increased number of cells in G2/M phase and cells in multipolar mitosis were observed even after 8 days of recovery. The presence of chrysotile fibers in the cell cultures was detected and cell morphology was observed by laser scanning confocal microscopy. After 4 and 8 days of recovery, only a few chrysotile fragments were present in some cells, and the cellular morphology was similar to that of control cells. Cells transfected with the GFP-tagged ±-tubulin plasmid were treated with chrysotile for 24 or 48 h and cells in multipolar mitosis were observed by time-lapse microscopy. Fates of these cells were established: retention in metaphase, cell death, progression through M phase generating more than two daughter cells or cell fusion during telophase or cytokinesis. Some of them were related to the formation of aneuploid cells and cells with abnormal number of centrosomes. (AU)