Prospecting volatile compounds produced by Brazilian cyanobacteria with allelopathic effects

• Processo: 11/51950-3
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
• Data de Início da vigência: 01 de julho de 2012
• Data de Término da vigência: 30 de junho de 2014
• Área do conhecimento: Ciências Exatas e da Terra - Química - Química Orgânica
• Acordo de Cooperação: Agilent
• Pesquisador responsável: Ernani Pinto Junior
• Beneficiário: Ernani Pinto Junior
• Instituição Sede: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Empresa: Agilent Technologies Brasil Ltda
• Município: São Paulo
• Assunto(s): Cianobactérias Biomarcadores Alelopatia Metabolômica Compostos voláteis
• Palavra(s)-Chave do Pesquisador: Allelopathy | Biomarkers | Cyanobacteria | Gc Ms | Metabolomics | Volatile Compounds

Resumo

Co-occurrence of toxic cyanobacteria blooming in different aquatic environments in several regions of Brazil (Northeast, Center east, Southeast and South) has already been registered. Among the negative consequences, the production of volatile organic compounds (VOCs) is of major importance. The most commonly VOCs are geosmin and 2-methylisoborneol, which resist to conventional water treatment procedures, causing odor issues, and can bioaccumulate in fish and shellfish. Moreover, allelopathic effects of VOCs in the aquatic community have been observed. In this regard, competition studies between cyanobacterial strains or between cyanobacteria and other organisms may shed light on the role played by VOCs in the environment. Thus, this study aims to evaluate the production of VOCs by different strains of cyanobacteria isolated from Brazilian reservoirs while developing effective analytical routines for their determination. The effect of environmental variables (light, temperature and pH) on the production of VOCs will also be evaluated. In order to investigate the possible allelopathic effects of these compounds, culture exudates will be tested in mixed cultivation flasks or in single species cultures. VOCs production will be followed by GC/MS employing methods developed for their correct identification. At the end, we aim to collect evidence over the production of VOCs and their effect on the physiologic parameters of cyanobacteria, providing data on the role played by these compounds and contributing to increase the scarce information currently available in Brazil and other countries. (AU)

Publicações científicas

(5)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

SANZ, MIRIAM; DOERR, FELIPE AUGUSTO; PINTO, ERNANI. First report of spumigin production by the toxic Sphaerospermopsis torques-reginae cyanobacterium. Toxicon, v. 108, p. 15-18, DEC 15 2015. (14/50420-9, 12/25272-0, 11/51950-3)

MORETTO, JESSICA A.; FREITAS, PALOMA N. N.; SOUZA, JULIANA P.; OLIVEIRA, THALITA M.; BRITES, ISABELLA; PINTO, ERNANI. Off-Flavors in Aquacultured Fish: Origins and Implications for Consumers. FISHES, v. 7, n. 1, p. 12-pg., 2022-02-01. (21/00149-0, 13/05969-0, 13/07914-8, 11/51950-3)

DE SOUZA, ALEXANDER OSSANES; TERAMOTO, KAZUMI KINOSHITA; DE FREITAS, PALOMA NATHANE NUNES; DE ALMEIDA, ERYKA COSTA; FERREIRA FILHO, SIDNEY SECKLER; PINTO, ERNANI. Effect of pre-chlorination on Microcystis aeruginosa cell integrity and microcystins removal. URBAN WATER JOURNAL, v. 20, n. 8, p. 11-pg., 2023-06-01. (11/51950-3, 10/50739-4)

SILVA MORETTO, JESSICA APARECIDA; NUNES DE FREITAS, PALOMA NATHANE; DE ALMEIDA, ERYKA COSTA; ALTARUGIO, LUCAS MIGUEL; DA SILVA, SIMONE VIEIRA; FIORE, MARLI DE FATIMA; PINTO, ERNANI. Effects of different cultivation conditions on the production of beta-cyclocitral and beta-ionone in Microcystis aeruginosa. BMC Microbiology, v. 22, n. 1, p. 13-pg., 2022-03-24. (21/00149-0, 13/05969-0, 13/07914-8, 11/51950-3)

SANZ, MIRIAM; DINI ANDREOTE, ANA PAULA; FIORE, MARLI FATIMA; DOERR, FELIPE AUGUSTO; PINTO, ERNANI. Structural Characterization of New Peptide Variants Produced by Cyanobacteria from the Brazilian Atlantic Coastal Forest Using Liquid Chromatography Coupled to Quadrupole Time-of-Flight Tandem Mass Spectrometry. MARINE DRUGS, v. 13, n. 6, p. 3892-3919, JUN 2015. (12/25272-0, 09/15402-1, 13/09192-0, 11/51950-3)