Functional genomics of photosynthetic genes in sugarcane

• Processo: 08/52066-7
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Data de Início da vigência: 01 de março de 2009
• Data de Término da vigência: 28 de fevereiro de 2014
• Área do conhecimento: Ciências Biológicas - Bioquímica - Biologia Molecular
• Pesquisador responsável: Helaine Carrer
• Beneficiário: Helaine Carrer
• Instituição Sede: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brasil
• Auxílio(s) vinculado(s): 13/50329-9 - Consequences of manipulation of the key enzyme of carbon dioxide assimilation, AP.R
• Bolsa(s) vinculada(s): 13/17474-5 - Transformação Genética de Cana-de-açúcar, BP.TT; 12/12268-5 - Análise de expressão e avaliação fenotípica de plantas de cana-de-açúcar transformadas geneticamente com o gene C4-PEPC, BP.IC; 11/17321-9 - Transformação genética de cana-de-açúcar, BP.TT; + mais bolsas vinculadas 11/02710-0 - Análises fisiológica, bioquímica e de expressão gênica em plantas de cana-de-açúcar transformadas geneticamente com o gene AtBI-1 submetidas ao déficit hídrico, BP.MS; 10/16541-2 - Transformação genética de cana de açúcar por Agrobacterium, BP.IC; 10/18485-2 - Genômica funcional de genes fotossintéticos em cana-de-açúcar: transformação nuclear de plantas, BP.TT; 10/12109-9 - Estudo Funcional de Genes Fotossintéticos em Cana-de-açúcar: Transformação de Plantas, BP.TT; 09/13662-6 - Genômica funcional de genes envolvidos na fotossíntese da cana-de-açúcar: Desenvolvimento de métodos de transformação nuclear e de plastídeos em cana-de-açúcar, BP.PD; 09/12048-2 - Genomica Funcional de Genes Fotossintéticos em Cana-de-Açúcar: Transformação Nuclear de Plantas, BP.TT; 09/13648-3 - Estudo Funcional de Genes Fotossintéticos em Cana-de-açúcar: Cultura de Tecidos, BP.TT - menos bolsas vinculadas
• Assunto(s): Fotossíntese Biologia computacional Expressão gênica Cloroplastos Cana-de-açúcar Bioenergia
• Palavra(s)-Chave do Pesquisador: Bioinformatics | Chloroplasts | Gene Expression | Photosynthesis | Plant Genetic Transformation | Transposale Elements
• Publicação FAPESP: https://media.fapesp.br/bv/uploads/publicacoes/pasta_bioen_jun2012_66.pdf

Resumo

Sugarcane (Saccharum officinarum) is one of the most important feedstock sources for biofuel. In Brazil, sugarcane has been a prominent cultivated species undergoing accelerated expansion. This crop has launched Brazil as the major and most relevant country for exporting ethanol, as well as it became an important source of world bioenergy. In order to sustain and develop this enlarging agricultural and commercial sector, in a long-term, it becomes mandatory a continued quick release of increasingly productive sugarcane cultivars carrying specific advantageous traits, including increased sucrose content. Adversely, the breeding of sugarcane has been naturally limited by its low fertility, complex genome, narrow genetic basis, and long periods of 12 to 15 years to create a new variety. The development of efficient systems of molecular biology and genetic transformation are fundamental, and often the only way, to rapidly introducing new valuable agronomic and commercial traits into sugarcane elite germplasm. Increase of sucrose content in elite sugarcane cultivars may be a main point to be addressed by using genetic transformation, and is directly dependent of increasing photosynthetic efficiency. The vast majority of photosynthetic proteins is nucleus-encoded and require N-terminal presequences, named chloroplast transit peptides, to target them to the chloroplast. About 2100 to 3600 distinct chloroplast proteins are nuclear-encoded, while about 100 to 120 are encoded by the organelle genome. The present project aims to develop efficient methods of sugarcane in vitro culture as well as methods of nuclear and chloroplast genetic transformation, applying them to modify photosynthetic genes in order to incorporate new photosynthetic traits in already productive Brazilian cultivars. The sugarcane photosynthetic efficiency is expected to be improved upon manipulation of photosynthetic genes (i.e. ribulose-1,5-bisphosphate carboxylase/oxygenase, phosphoenolpyruvate carboxylase, carbonic anhydrase) generating novel knowledge in this research field as well as leading to increased synthesis of triose phosphates and, ultimately, increased sucrose content in the transgenic cultivars. (AU)

Publicações científicas

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

RAMIRO, DANIEL ALVES; MELOTTO-PASSARIN, DANILA MONTEWKA; BARBOSA, MARIANA DE ALMEIDA; DOS SANTOS, FLAVIO; PEREZ GOMEZ, SERGIO GREGORIO; MASSOLA JUNIOR, NELSON SIDNEI; LAM, ERIC; CARRER, HELAINE. Expression of Arabidopsis Bax Inhibitor-1 in transgenic sugarcane confers drought tolerance. Plant Biotechnology Journal, v. 14, n. 9, p. 1826-1837, SEP 2016. (08/52066-7)