Integrated enzyme discovery and pathway optimization for bagase valorization

• Processo: 14/50371-8
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Data de Início da vigência: 01 de julho de 2014
• Data de Término da vigência: 31 de dezembro de 2016
• Área do conhecimento: Ciências Biológicas - Bioquímica
• Acordo de Cooperação: BBSRC, UKRI
• Pesquisador responsável: Fábio Márcio Squina
• Beneficiário: Fábio Márcio Squina
• Pesquisador Responsável no exterior: Neil Dixon
• Instituição Parceira no exterior: University of Manchester, Inglaterra
• Instituição Sede: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brasil
• Assunto(s): Enzimas Metagenômica Biologia sintética Bioprospecção Bioenergia
• Palavra(s)-Chave do Pesquisador: Bioproducts | Enzymes | Metagenomcs | Synthetic Biology
• Publicação FAPESP: https://media.fapesp.br/bv/uploads/pdfs/fapesp_uk_9Mji1ST_26_26.pdf

Resumo

The ultimate aims of this project are i) to utilize a microbial metagenomics platform to enable novel enzyme building block discovery and identification, ii) to assess the capabilities and of the bacteriallignin peroxidase treatment against sugarcane bagasse lignin, iii) to integrate these building blocks into suitably engineered host chassis using synthetic biology principies and to engineer convergent biosynthetic pathways to valorized lignin using multiple feedstocks, iv) to assess these integrated recombinant systems under high-density cell fermentation, using bioprocess analysis to optimize pathway flux and process design space. The pump-priming project will contain a number of activities focused on collaboration building and partner integration, and a number of praclical activities focused on gathering preliminary proof of concept experimental data. (AU)

Publicações científicas

(4)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

MANDELLI, FERNANDA; RAMIRES, BRENDA OLIVEIRA; COUGER, MATTHEW BRIAN; PAIXAO, DOUGLAS A. A.; CAMILO, CESAR M.; POLIKARPOV, IGOR; PRADE, ROLF; RIANO-PACHON, DIEGO M.; SQUINA, FABIO M.. Draft Genome Sequence of the Thermophile Thermus filiformis ATCC 43280, Producer of Carotenoid-(Di)glucoside-Branched Fatty Acid (Di)esters and Source of Hyperthermostable Enzymes of Biotechnological Interest. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 3, MAY-JUN 2015. (14/50371-8)

FRANCO CAIRO, JOAO P. L.; CARAZZOLLE, MARCELO F.; LEONARDO, FLAVIA C.; MOFATTO, LUCIANA S.; BRENELLI, LIVIA B.; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE A.; DOMINGUES, ROMANIA R.; ALVAREZ, THABATA M.; TRAMONTINA, ROBSON; et al. Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi. FRONTIERS IN MICROBIOLOGY, v. 7, OCT 13 2016. (15/06971-3, 06/59086-8, 14/20576-7, 14/50371-8, 12/19040-0, 14/10351-8, 11/20977-3, 13/03061-0, 10/11469-1, 08/50114-4, 08/58037-9)

BRENELLI, LIVIA BEATRIZ; MANDELLI, FERNANDA; MERCADANTE, ADRIANA ZERLOTTI; DE MORAES ROCHA, GEORGE JACKSON; ROCCO, SILVANA APARECIDA; CRAIEVICH, ALDO FELIX; GONCALVES, ADILSON ROBERTO; CENTENO, DANILO DA CRUZ; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO MARCIO. Acidification treatment of lignin from sugarcane bagasse results in fractions of reduced polydispersity and high free-radical scavenging capacity. INDUSTRIAL CROPS AND PRODUCTS, v. 83, p. 94-103, MAY 2016. (13/03061-0, 14/50371-8)

MANDELLI, FERNANDA; RAMIRES, BRENDA OLIVEIRA; COUGER, MATTHEW BRIAN; PAIXAO, DOUGLAS A. A.; CAMILO, CESAR M.; POLIKARPOV, IGOR; PRADE, ROLF; RIANO-PACHON, DIEGO M.; SQUINA, FABIO M.. Draft Genome Sequence of the Thermophile Thermus filiformis ATCC 43280, Producer of Carotenoid-(Di)glucoside-Branched Fatty Acid (Di)esters and Source of Hyperthermostable Enzymes of Biotechnological Interest. GENOME ANNOUNCEMENTS, v. 3, n. 3, p. 2-pg., 2015-05-01. (14/50371-8)