Integrated enzyme discovery and pathway optimization for bagase valorization

Resumo

The ultimate aims of this project are i) to utilize a microbial metagenomics platform to enable novel enzyme building block discovery and identification, ii) to assess the capabilities and of the bacteriallignin peroxidase treatment against sugarcane bagasse lignin, iii) to integrate these building blocks into suitably engineered host chassis using synthetic biology principies and to engineer convergent biosynthetic pathways to valorized lignin using multiple feedstocks, iv) to assess these integrated recombinant systems under high-density cell fermentation, using bioprocess analysis to optimize pathway flux and process design space. The pump-priming project will contain a number of activities focused on collaboration building and partner integration, and a number of praclical activities focused on gathering preliminary proof of concept experimental data. (AU)