Integrated enzyme discovery and pathway optimization for bagase valorization

• Grant number: 14/50371-8
• Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Duration: July 01, 2014 - December 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry
• Cooperation agreement: BBSRC, UKRI
• Principal researcher: Fábio Márcio Squina
• Grantee: Fábio Márcio Squina
• Principal researcher abroad: Neil Dixon
• Institution abroad: University of Manchester, England
• Home Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil

Abstract

The ultimate aims of this project are i) to utilize a microbial metagenomics platform to enable novel enzyme building block discovery and identification, ii) to assess the capabilities and of the bacteriallignin peroxidase treatment against sugarcane bagasse lignin, iii) to integrate these building blocks into suitably engineered host chassis using synthetic biology principies and to engineer convergent biosynthetic pathways to valorized lignin using multiple feedstocks, iv) to assess these integrated recombinant systems under high-density cell fermentation, using bioprocess analysis to optimize pathway flux and process design space. The pump-priming project will contain a number of activities focused on collaboration building and partner integration, and a number of praclical activities focused on gathering preliminary proof of concept experimental data. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCO CAIRO, JOAO P. L.; CARAZZOLLE, MARCELO F.; LEONARDO, FLAVIA C.; MOFATTO, LUCIANA S.; BRENELLI, LIVIA B.; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE A.; DOMINGUES, ROMANIA R.; ALVAREZ, THABATA M.; TRAMONTINA, ROBSON; VIDAL, RAMON O.; COSTA, FERNANDO F.; COSTA-LEONARDO, ANA M.; PAES LEME, ADRIANA F.; PEREIRA, GONCALO A. G.; SQUINA, FABIO M. Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi. FRONTIERS IN MICROBIOLOGY, v. 7, OCT 13 2016. Web of Science Citations: 8.

BRENELLI, LIVIA BEATRIZ; MANDELLI, FERNANDA; MERCADANTE, ADRIANA ZERLOTTI; DE MORAES ROCHA, GEORGE JACKSON; ROCCO, SILVANA APARECIDA; CRAIEVICH, ALDO FELIX; GONCALVES, ADILSON ROBERTO; CENTENO, DANILO DA CRUZ; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO MARCIO. Acidification treatment of lignin from sugarcane bagasse results in fractions of reduced polydispersity and high free-radical scavenging capacity. INDUSTRIAL CROPS AND PRODUCTS, v. 83, p. 94-103, MAY 2016. Web of Science Citations: 10.

MANDELLI, FERNANDA; RAMIRES, BRENDA OLIVEIRA; COUGER, MATTHEW BRIAN; PAIXAO, DOUGLAS A. A.; CAMILO, CESAR M.; POLIKARPOV, IGOR; PRADE, ROLF; RIANO-PACHON, DIEGO M.; SQUINA, FABIO M. Draft Genome Sequence of the Thermophile Thermus filiformis ATCC 43280, Producer of Carotenoid-(Di)glucoside-Branched Fatty Acid (Di)esters and Source of Hyperthermostable Enzymes of Biotechnological Interest. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 3 MAY-JUN 2015. Web of Science Citations: 0.