Transcriptome Sequencing and Developmental Regulation of Gene Expression in Anopheles aquasalis

• Processo: 14/12874-8
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Data de Início da vigência: 01 de agosto de 2014
• Data de Término da vigência: 31 de janeiro de 2015
• Área do conhecimento: Ciências Biológicas - Parasitologia - Entomologia e Malacologia de Parasitos e Vetores
• Pesquisador responsável: Margareth de Lara Capurro-Guimarães
• Beneficiário: Margareth de Lara Capurro-Guimarães
• Instituição Sede: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Assunto(s): Malária Expressão gênica
• Palavra(s)-Chave do Pesquisador: Anopheles aquasalis | expressão gênica | malária | transcriptoms | Expressao genica de vetores

Resumo

Anopheles aquasalis is a major malaria vector in coastal areas of Southand Central America where it breeds preferentially in brackish water. This species isvery susceptible to Plasmodium vivax and it has been already incriminated asresponsible vector in malaria outbreaks. There has been no high-throughputinvestigation into the sequencing of An. aquasalis genes, transcripts and proteinsdespite its epidemiological relevance. Here we describe the sequencing, assembly andannotation of the An. aquasalis transcriptome.Methodology/Principal Findings:A total of 419 thousand cDNA sequence reads,encompassing 164 million nucleotides, were assembled in 7544 contigs of e 2sequences, and 1999 singletons. The majority of the An. aquasalis transcripts encodeproteins with their closest counterparts in another neotropical malaria vector,An.Background:Anopheles aquasalis is a major malaria vector in coastal areas of Southand Central America where it breeds preferentially in brackish water. This species isvery susceptible to Plasmodium vivax and it has been already incriminated asresponsible vector in malaria outbreaks. There has been no high-throughputinvestigation into the sequencing of An. aquasalis genes, transcripts and proteinsdespite its epidemiological relevance. Here we describe the sequencing, assembly andannotation of the An. aquasalis transcriptome.Methodology/Principal Findings:A total of 419 thousand cDNA sequence reads,encompassing 164 million nucleotides, were assembled in 7544 contigs of e 2sequences, and 1999 singletons. The majority of the An. aquasalis transcripts encodeproteins with their closest counterparts in another neotropical malaria vector,An.Background:Anopheles aquasalis is a major malaria vector in coastal areas of Southand Central America where it breeds preferentially in brackish water. This species isvery susceptible to Plasmodium vivax and it has been already incriminated asresponsible vector in malaria outbreaks. There has been no high-throughputinvestigation into the sequencing of An. aquasalis genes, transcripts and proteinsdespite its epidemiological relevance. Here we describe the sequencing, assembly andannotation of the An. aquasalis transcriptome.Methodology/Principal Findings:A total of 419 thousand cDNA sequence reads,encompassing 164 million nucleotides, were assembled in 7544 contigs of e 2sequences, and 1999 singletons. The majority of the An. aquasalis transcripts encodeproteins with their closest counterparts in another neotropical malaria vector,An.Background:Anopheles aquasalis is a major malaria vector in coastal areas of Southand Central America where it breeds preferentially in brackish water. This species isvery susceptible to Plasmodium vivax and it has been already incriminated asresponsible vector in malaria outbreaks. There has been no high-throughputinvestigation into the sequencing of An. aquasalis genes, transcripts and proteinsdespite its epidemiological relevance. Here we describe the sequencing, assembly andannotation of the An. aquasalis transcriptome.Methodology/Principal Findings:A total of 419 thousand cDNA sequence reads,encompassing 164 million nucleotides, were assembled in 7544 contigs of e 2sequences, and 1999 singletons. The majority of the An. aquasalis transcripts encodeproteins with their closest counterparts in another neotropical malaria vector,An.Background:Anopheles aquasalis is a major malaria vector in coastal areas of Southand Central America where it breeds preferentially in brackish water. This species isvery susceptible to Plasmodium vivax and it has been already incriminated asresponsible vector in malaria outbreaks. There has been no high-throughputinvestigation into the sequencing of An. aquasalis genes, transcripts and proteinsdespite its epidemiological relevance. Here we describe the sequencing, assembly andannotation of the An. aquasalis transcriptome (AU)