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Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats

Processo: 14/16535-3
Linha de fomento:Auxílio à Pesquisa - Publicações científicas - Artigo
Vigência: 01 de setembro de 2014 - 28 de fevereiro de 2015
Área do conhecimento:Ciências Biológicas - Fisiologia - Fisiologia Geral
Pesquisador responsável:Beatriz de Oliveira Monteiro
Beneficiário:Beatriz de Oliveira Monteiro
Instituição-sede: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brasil
Assunto(s):Neurofisiologia  Transplantes  Hipocampo  Ansiedade 


The GABAergic system plays an important role in modulating levels of anxiety. Whentransplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) havethe ability to differentiate into GABAergic interneurons and modify the inhibitory tone in thehost brain. Currently, two methods have been reported for obtaining MGE precursor cells fortransplantation: fresh and neurosphere dissociated cells. Here, we investigated the effectsgenerated by transplantation of the two types of cell preparations on anxiety behavior in rats. Results We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain ofmale rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animalswere tested in the Elevated Plus Maze (EPM). To verify the differentiation and migrationpattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry forGFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) andcalretinin (CR). Cells from both types of preparations expressed these interneuronal markers.However, an anxiolytic effect on behavior in the EPM was observed in animals that receivedthe MGE-derived freshly dissociated cells but not in those that received the neurospheredissociated cells. Conclusion Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cellsthat reinforces the inhibitory function of the GABAergic neuronal circuitry in thehippocampus related to anxiety-like behavior in rats. (AU)