Study of the mutagenic activity of azo dyes processing plants effluent - which compounds should to be regulated in the aquatic environment?

• Processo: 01/14521-5
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Data de Início da vigência: 01 de setembro de 2002
• Data de Término da vigência: 31 de janeiro de 2003
• Área de conhecimento: Ciências Biológicas - Genética - Mutagênese
• Pesquisador responsável: Gisela de Aragão Umbuzeiro
• Beneficiário: Gisela de Aragão Umbuzeiro
• Pesquisador Anfitrião: David Demarini
• Instituição Sede: Companhia Ambiental do Estado de São Paulo (CETESB). São Paulo , SP, Brasil
• Instituição Anfitriã: United States Environmental Protection Agency (EPA), Estados Unidos
• Assunto(s): Carcinógenos Aminas Poluição ambiental Corantes Genotoxicidade Testes de mutagenicidade
• Palavra(s)-Chave do Pesquisador: Aminas Aromaticas | Compostos Cancerigenos | Contaminacao Ambiental | Corantes | Genotoxicidade | Mutagenicidade

Resumo

Dyes used in industries that presented high mutagenic effluent in the past will be selected. The process of dyeing will be simulated and the resultant complex mixtures will be tested in the Salmonella microsome/mutagenicity test, the comet assay and the formation of DNA adducts.. Chemical characterization will be performed using HPLC/MS. A pilot study will be conducted. The compounds suspected to be responsible for the mutagenic activity observed in the complex mixture will be tested in the same bioassays. As a consequence, standards will be proposed to adequately regulate mutagenic compounds originated from dyeing plant discharges in the aquatic environment. (AU)