Study of the mutagenic activity of azo dyes processing plants effluent - which compounds should to be regulated in the aquatic environment?

• Processo: 01/14521-5
• Linha de fomento: Bolsas no Exterior - Pesquisa
• Vigência (Início): 01 de setembro de 2002
• Vigência (Término): 31 de janeiro de 2003
• Área do conhecimento: Ciências Biológicas - Genética - Mutagênese
• Pesquisador responsável: Gisela de Aragão Umbuzeiro
• Beneficiário: Gisela de Aragão Umbuzeiro
• Anfitrião: David Demarini
• Instituição-sede: Companhia Ambiental do Estado de São Paulo (CETESB). Secretaria do Meio Ambiente (São Paulo - Estado). São Paulo , SP, Brasil
• Local de pesquisa : United States Environmental Protection Agency (EPA), Estados Unidos
• Assunto(s): Carcinógenos Aminas Poluição ambiental Corantes Genotoxicidade Testes de mutagenicidade

Resumo

Dyes used in industries that presented high mutagenic effluent in the past will be selected. The process of dyeing will be simulated and the resultant complex mixtures will be tested in the Salmonella microsome/mutagenicity test, the comet assay and the formation of DNA adducts.. Chemical characterization will be performed using HPLC/MS. A pilot study will be conducted. The compounds suspected to be responsible for the mutagenic activity observed in the complex mixture will be tested in the same bioassays. As a consequence, standards will be proposed to adequately regulate mutagenic compounds originated from dyeing plant discharges in the aquatic environment. (AU)