Green tea extract improves high fat diet-induced hypothalamic inflammation, without affecting the serotoninergic system

Autor(es)

Okuda, Marcos H. ^[1] ; Zemdegs, Juliane C. S. ^[1] ; de Santana, Aline A. ^[1] ; Santamarina, Aline B. ^[1] ; Moreno, Mayara F. ^[1] ; Hachul, Ana C. L. ^[1] ; dos Santos, Bruno ^[1] ; Oller do Nascimento, Claudia M. ^[1] ; Ribeiro, Eliane B. ^[1] ; Oyama, Lila M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Disciplina Fisiol Nutr, Dept Fisiol, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 25, n. 10, p. 1084-1089, OCT 2014.
• Citações Web of Science: 18

Resumo

To investigate possible mechanisms of green tea's anti-obesity and anti-diabetic effects in the hypothalamus, the central regulator of metabolism, of mice fed with high-fat diet (HFD), we analyzed proteins of the toll-like receptor 4 (TLR4) pathway and serotoninergic proteins involved in energy homeostasis. Thirty-day-old male Swiss mice were fed with HFD rich in saturated fat and green tea extract (GTE) for 8 weeks. After that, body weight and mass of fat depots were evaluated. Oral glucose tolerance test was performed 3 days prior to euthanasia; serum glucose, insulin and adiponectin were measured in fasted mice. Hypothalamic TLR4 pathway proteins, serotonin receptors 1B and 2C and serotonin transporter were analyzed by Western blotting or enzyme-linked immunosorbent assay. A second set of animals was used to measure food intake in response to fluoxetine, a selective serotonin reuptake inhibitor. Mice fed with HFD had increased body weight and mass of fat depots, impaired oral glucose tolerance, elevated glucose and insulin and decreased adiponectin serum levels. TLR4, I kappa B-alpha, nuclear factor kappa B p50 and interleukin 6 were increased by HFD. Concomitant GTE treatment ameliorated these parameters. The serotoninergic system remained functional after HFD treatment despite a few alterations in protein content of serotonin receptors I B and 2C and serotonin transporter. In summary, the GTE attenuated the deleterious effects of the HFD investigated in this study, partially due to reduced hypothalamic inflammation. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/03713-5 - Efeito da ingestão de um extrato de chá verde rico em epigalocatequina-3-galato na expressão de citocinas e reguladores metabólicos no tecido adiposo e músculo esquelético de camundongos obesos induzidos por dieta hiperlipídica
• Beneficiário: Lila Missae Oyama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/16199-5 - Efeito do tratamento prolongado com galato-3-epigalocatequina sobre o teor hipotalâmico e hipocampal de proteínas relacionadas aos sistemas serotoninérgico e inflamatório.
• Beneficiário: Marcos Hiromu Okuda
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/14373-8 - Estudo dos efeitos da galato-3-epigalocatequina sobre a secreção de adipocinas, a via de sinalização do TLR4 e do NF-°B em adipócitos: estudo in vivo e in vitro
• Beneficiário: Lila Missae Oyama
• Modalidade de apoio: Auxílio à Pesquisa - Regular